bacto_marine_broth_difco_2216_for_dsm_11879 (CultureMech:008906)

Overview

External ontology

TOGO:M2319 — Bacto Marine Broth (Difco 2216) (For DSM 11879)

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2319 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514.pdf

Composition

flowchart LR
    CultureMech_008906["bacto_marine_broth_difco_2216_for_dsm_11879"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008906 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_008906 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_H3BO3["H3BO3"]
    CultureMech_008906 --> ing2_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing2_H3BO3 -.-> CHEBI_33118
    ing3_KCl["KCl"]
    CultureMech_008906 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaHCO3["NaHCO3"]
    CultureMech_008906 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Na2SO4["Na2SO4"]
    CultureMech_008906 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_CaCl2["CaCl2"]
    CultureMech_008906 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_Na2HPO4["Na2HPO4"]
    CultureMech_008906 --> ing7_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing7_Na2HPO4 -.-> CHEBI_34683
    ing8__NH4_NO3["(NH4)NO3"]
    CultureMech_008906 --> ing8__NH4_NO3
    CHEBI_63038["CHEBI:63038"]
    ing8__NH4_NO3 -.-> CHEBI_63038
    ing9_NaF["NaF"]
    CultureMech_008906 --> ing9_NaF
    CHEBI_28741["CHEBI:28741"]
    ing9_NaF -.-> CHEBI_28741
    ing10_KBr["KBr"]
    CultureMech_008906 --> ing10_KBr
    CHEBI_32030["CHEBI:32030"]
    ing10_KBr -.-> CHEBI_32030
    ing11_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_008906 --> ing11_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing11_Fe_III__citrate -.-> CHEBI_144421
    ing12_SrCl2["SrCl2"]
    CultureMech_008906 --> ing12_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing12_SrCl2 -.-> CHEBI_36383
    ing13_Na_silicate["Na-silicate"]
    CultureMech_008906 --> ing13_Na_silicate
    CHEBI_60720["CHEBI:60720"]
    ing13_Na_silicate -.-> CHEBI_60720
    ing14_MgCl2__anhydrous_["MgCl2 (anhydrous)"]
    CultureMech_008906 --> ing14_MgCl2__anhydrous_
    ing15_Bacto_yeast_extract["Bacto yeast extract"]
    CultureMech_008906 --> ing15_Bacto_yeast_extract
    ing16_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_008906 --> ing16_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing16_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing17_Bacto_peptone["Bacto peptone"]
    CultureMech_008906 --> ing17_Bacto_peptone

Ingredients (18)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1000 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2	NaCl Cl.Na 58.443 g/mol	19.45 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	H3BO3 H3BO3 61.833 g/mol	22 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	KCl Cl.K 74.551 g/mol	0.55 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5	NaHCO3 CHO3.Na 84.006 g/mol	0.16 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6	Na2SO4 2Na.O4S 142.043 g/mol	3.24 G_PER_L	CHEBI:32149 sodium sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7	CaCl2 Ca.2Cl 110.984 g/mol	1.8 G_PER_L	CHEBI:3312 calcium dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8	Na2HPO4 HO4P.2Na 141.958 g/mol	8 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9	(NH4)NO3 H4N.NO3 80.043 g/mol	1.6 G_PER_L	CHEBI:63038 ammonium nitrate	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
10	NaF F.Na 41.988 g/mol	2.4 G_PER_L	CHEBI:28741 sodium fluoride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11	KBr Br.K 119.002 g/mol	0.08 G_PER_L	CHEBI:32030 potassium bromide	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12	Fe(III) citrate C6H5FeO7 244.944 g/mol	0.1 G_PER_L	CHEBI:144421 iron(III) citrate	Role: Mineral source; Properties: Organic compound, Defined component, Simple component
13	SrCl2 2Cl.Sr 158.526 g/mol	34 G_PER_L	CHEBI:36383 strontium dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14	Na-silicate 2Na.O3Si 122.063 g/mol	4 G_PER_L	CHEBI:60720 sodium silicate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15	MgCl2 (anhydrous)	5.9 G_PER_L		Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16	Bacto yeast extract	1 G_PER_L		Properties: Undefined component
17	Na2S2O3 x 5 H2O 5H2O.2Na.O3S2 248.186 g/mol	1 G_PER_L	CHEBI:32150 sodium thiosulfate pentahydrate	Properties: Inorganic compound, Simple component, Defined component
18	Bacto peptone	5 G_PER_L		Properties: Undefined component

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Cellulophaga algicola
Term
Id
NCBITaxon:59600
Label
Cellulophaga algicola
Strain
IC166T
Evidence
- Reference
  doi:10.4056/sigs.1543845
  Supports
  SUPPORT
  Explanation
  Abt et al. 2011 genome report; explicit type-strain growth on DSMZ medium 514 (= this parent medium) at 15C. | Identifiers: DSM 14237; CIP 107446; LMG 21425; INSDC CP002453; BioProject 41529; IMG 2503904003
  Snippet
  C. algicola IC166T, DSM 14237, was grown in DSMZ medium 514 (BACTO marine broth) at 15C.
Preferred term
Kangiella koreensis
Term
Id
NCBITaxon:261964
Label
Kangiella koreensis
Strain
SW-125T
Evidence
- Reference
  doi:10.4056/sigs.36635
  Supports
  SUPPORT
  Explanation
  Han et al. 2009; direct cultivation/growth testing of the type strain on Marine Agar/Broth 2216 (= this parent medium family). | Identifiers: DSM 16069; KCTC 12182; 16S AY520560
  Snippet
  Colony morphology and temperature growth were determined on marine agar 2216 (Difco), and pH dependence was determined in marine broth 2216 (Difco). Colonies after 7 d at 30C; optimum 30-37C; pH optimum 7.0-8.0.
Preferred term
Phaeobacter caeruleus
Strain
DSM 24564T
Evidence
- Reference
  doi:10.4056/sigs.3927626
  Supports
  SUPPORT
  Explanation
  Beyersmann et al. 2013; explicit growth on DSMZ medium 514 / Marine Broth 2216 at 20C.
  Snippet
  P. caeruleus DSM 24564T was grown in DSMZ medium 514. After incubation for 24 h in marine broth 2216 with shaking, visible growth was observed at 20C but not at 28C (100 rpm).
Preferred term
Roseobacter ponti
Term
Id
NCBITaxon:1891787
Label
Roseobacter ponti
Strain
DSM 106830T
Evidence
- Reference
  doi:10.1093/gbe/evaa114
  Supports
  SUPPORT
  Explanation
  Hollensteiner et al. 2020; medium identity explicitly aliased to this parent record; 30C, 180 rpm, 2 d. | Identifiers: DSM 106830T; KCTC 52469T; NBRC 112431T
  Snippet
  A single colony was passed for 2 days in Medium 514, explicitly identified as DSMZ Medium 514 Bacto Marine Broth Difco 2216, at 30C and 180 rpm.
Preferred term
Joostella marina
Strain
En5T
Evidence
- Reference
  doi:10.4056/sigs.3537045
  Supports
  SUPPORT
  Explanation
  Stackebrandt et al. 2013; isolation on Marine Agar 2216 (recognizable 2216 formulation under this parent medium family). Agar isolation rather than broth growth. | Identifiers: DSM 19592; KCTC 12518; CGMCC 1.6973
  Snippet
  J. marina strain En5T was isolated by dilution-plating on marine agar 2216 (Difco).
Preferred term
Rhodothermus profundi
Term
Id
NCBITaxon:633813
Label
Rhodothermus profundi
Strain
PRI 2902T
Evidence
- Reference
  doi:10.1099/ijs.0.012724-0
  Supports
  SUPPORT
  Explanation
  Marteinsson et al. 2010; type strain grown on the half-strength MB1/2 variant of this parent medium (see variant mb_half_strength_marine_broth_2216). Deep-sea isolate; pressure tests at 0.1 and 25 MPa. | Identifiers: DSM 22212T; JCM 15944T
  Snippet
  MB1/2 defined as DSMZ medium 514 / Bacto Marine Broth Difco 2216 diluted to half strength with water; PRI 2902T grown on agar MB1/2 and in liquid MB1/2; colonies after 4 d at 70C.

Recipe variants

Name
mb_half_strength_marine_broth_2216
Relationship
CONCENTRATION_VARIANT
Description
MB1/2: DSMZ medium 514 / Bacto Marine Broth (Difco 2216) diluted to half strength with water, used for Rhodothermus profundi and related PRI isolates.
Modifications
- Marine broth/agar 2216 (DSMZ medium 514) diluted to 0.5x strength with water.
- For single-carbon-source assays, peptone and yeast extract omitted from MB1/2 plates (assay variant, not routine MB1/2).
Purpose
Half-strength marine broth/agar 2216 used to cultivate slow-growing deep-sea Rhodothermus isolates; recognizable concentration variant, not a separate medium.
Evidence
- Reference
  doi:10.1099/ijs.0.012724-0
  Supports
  SUPPORT
  Explanation
  Marteinsson et al. 2010 defines MB1/2 explicitly as half-strength DSMZ 514 / Bacto Marine Broth 2216.
  Snippet
  MB1/2 ... diluted to half strength with water; growth on single carbon sources tested on plates containing MB1/2 without peptone and yeast extract.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.333180Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2319
2026-01-28T19:48:22.784090Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.184316Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008906
2026-03-17T06:30:13.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.890738+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Bacto Marine Broth (Difco 2216) (For DSM 11879)" to "bacto_marine_broth_difco_2216_for_dsm_11879" for consistent matching
2026-05-28T06:12:21.617965+00:00	deep-research-edison-phase2	ENRICHED	Edison phase-1/phase-2: multiple type strains grown directly on DSMZ medium 514 / Marine Broth 2216 (parent); MB1/2 half-strength is a concentration variant. [added 6 organism(s), 1 variant(s)]
2026-06-05T07:40:50.450802+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.778382+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T00:58:02.688443+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 6 specific strain(s) named, no community signal (#142)