Overview
- External ontology
- TOGO:M2321 — Natronobacterium Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2321 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium205.pdf
Composition
flowchart LR
CultureMech_008909["natronobacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008909 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008909 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008909 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_008909 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Na3_citrate_x_2_H2O["Na3-citrate x 2 H2O"]
CultureMech_008909 --> ing4_Na3_citrate_x_2_H2O
CHEBI_32142["CHEBI:32142"]
ing4_Na3_citrate_x_2_H2O -.-> CHEBI_32142
ing5_Glutamic_acid["Glutamic acid"]
CultureMech_008909 --> ing5_Glutamic_acid
CHEBI_18237["CHEBI:18237"]
ing5_Glutamic_acid -.-> CHEBI_18237
ing6_Agar["Agar"]
CultureMech_008909 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Casamino_acids["Casamino acids"]
CultureMech_008909 --> ing7_Casamino_acids
ing8_Na2CO3["Na2CO3"]
CultureMech_008909 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
250 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
Na3-citrate x 2 H2O
C6H5O7.2H2O.3Na 294.099 g/mol
|
3 G_PER_L |
CHEBI:32142
sodium citrate dihydrate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 6 |
Glutamic acid
C5H9NO4 147.13 g/mol
|
2.5 G_PER_L |
CHEBI:18237
glutamic acid
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Casamino acids | 15 G_PER_L | Properties: Undefined component | |
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
variable VARIABLE |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.337750Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2321 |
| 2026-01-28T19:48:19.781097Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 |
| 2026-03-13T06:20:01.212504Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008909 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.922047+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natronobacterium Medium" to "natronobacterium_medium" for consistent matching |
| 2026-05-16T09:03:13.012352+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.206892+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.057611+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:26.125533+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.841974+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |