sulfolobus_medium_for_dsm_9790 (CultureMech:008911)

Overview

External ontology

TOGO:M2323 — Sulfolobus Medium (For DSM 9790)

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2323 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf

Composition

flowchart LR
    CultureMech_008911["sulfolobus_medium_for_dsm_9790"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008911 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008911 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_008911 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008911 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008911 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_008911 --> ing5_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing6_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
    CultureMech_008911 --> ing6_Yeast_extract__OXOID_
    ing7_H2SO4["H2SO4"]
    CultureMech_008911 --> ing7_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing7_H2SO4 -.-> CHEBI_26836
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_008911 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_008911 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_008911 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_008911 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing12_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_008911 --> ing12_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing12_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing13_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_008911 --> ing13_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing13_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing14_CoSO4["CoSO4"]
    CultureMech_008911 --> ing14_CoSO4
    CHEBI_53470["CHEBI:53470"]
    ing14_CoSO4 -.-> CHEBI_53470
    ing15_HCl["HCl"]
    CultureMech_008911 --> ing15_HCl
    CHEBI_17883["CHEBI:17883"]
    ing15_HCl -.-> CHEBI_17883

Ingredients (16)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1000.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1000.0, 1000.0]
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.25 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.07 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	KH2PO4 H2O4P.K 136.084 g/mol	0.28 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.3 G_PER_L	CHEBI:62946 ammonium sulfate	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.02 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7	Yeast extract (OXOID)	2 G_PER_L		Properties: Undefined component, Complex component
8	H2SO4 H2O4S 98.079 g/mol	variable VARIABLE	CHEBI:26836 sulfuric acid	Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
9	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	3 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	180 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	22 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	5 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13	VOSO4 x 2 H2O 2H2O.O4S.OV 199.036 g/mol	3 G_PER_L	CHEBI:87009 vanadyl sulfate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14	Na2B4O7 x 10 H2O 10H2O.H4B4O9.2Na 417.401 g/mol	450 G_PER_L	CHEBI:131366 disodium tetraborate decahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15	CoSO4 Co.O4S 154.996 g/mol	1 G_PER_L	CHEBI:53470 cobalt(2+) sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16	HCl HCl 36.461 g/mol	variable VARIABLE	CHEBI:17883 hydrogen chloride	Role: Solvating media; Properties: Defined component, Solution, Inorganic compound

Solutions

Allen’s trace element solution

Dose: 10 G_PER_L

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Picrophilus oshimae
Term
Id
NCBITaxon:46632
Label
Picrophilus oshimae
Strain
DSM 9790 (KAW 2/3, JCM 10055, NBRC 100828)
Evidence
- Reference
  https://www.dsmz.de/collection/catalogue/details/culture/DSM-9790
  Supports
  SUPPORT
  Explanation
  DSMZ catalogue entry for DSM 9790, relayed by BacDive strain record 11901 (bacdive.dsmz.de/strain/11901), which lists culture medium 'SULFOLOBUS MEDIUM (DSMZ Medium 88)' with growth: yes at 55 C and links to mediadive.dsmz.de/medium/88. That is the medium this record transcribes (TOGO M2323 -> DSMZ_Medium88.pdf), and DSM 9790 is the strain the record is named for. TAXONOMY: DSM 9790 was described as the Picrophilus torridus type strain (Zillig et al. 1996), but NCBI has since synonymized P. torridus with P. oshimae - NCBITaxon:46632 carries 'Picrophilus torridus' as a related synonym, and the former strain id NCBITaxon:263820 ('Picrophilus torridus DSM 9790') is now an alternative id of NCBITaxon:1122961. Curated to the current species term, with the strain designation preserved above.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.341767Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2323
2026-01-28T19:48:23.542911Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: HCl
2026-03-09T08:04:39.152848+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T03:09:08.990657Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:20:01.233983Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008911
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:15.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.944916+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Sulfolobus Medium (For DSM 9790)" to "sulfolobus_medium_for_dsm_9790" for consistent matching
2026-05-16T09:04:32.801256+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:41:47.456636+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:33.354387+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:12.022634+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-07-29T00:40:35.069219Z	provenance-review	ANNOTATED	Added Picrophilus oshimae strain DSM 9790, per the DSMZ catalogue entry (via BacDive record 11901): DSMZ Medium 88, growth yes, 55 C. Resolves issue #119 as no-rename - 'Sulfolobus Medium' is DSMZ's own name for Medium 88 and '(For DSM 9790)' is accurate TOGO provenance. Metallosphaera sedula deliberately NOT added here: it belongs to the sibling record CultureMech:008908 'Sulfolobus Medium (For DSM 5348)' (TOGO M2320), where it is already curated.
2026-08-06T00:58:02.597066+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 1 specific strain(s) named, no community signal (#142)
2026-09-02T07:37:31.983404+00:00	repair_merged_duplicates.py	REPAIRED_SUMMED_DUPLICATE_MERGE	Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.