cytophaga_hutchinsonii_medium (CultureMech:008924)

Overview

External ontology

TOGO:M2337 — Cytophaga Hutchinsonii Medium

Applications

Microbial cultivation

pH

7.2

Notes

TOGO M2337 imports DSMZ Medium 160 as Cytophaga Hutchinsonii Medium. DSMZ Medium 160 adds 0.5% filter-sterilized cellobiose to DSMZ Medium 67. DSMZ Medium 67 lists, per liter, 3 g Casitone, 1.36 g CaCl2 x 2 H2O, 1 g Yeast extract, and 1000 ml Distilled water; the imported M2337 branch is represented without the 15 g agar from DSMZ Medium 67 and with 0.5% w/v Cellobiose converted to 5 g/L. Adjust pH to 7.2.

Composition

flowchart LR
    CultureMech_008924["cytophaga_hutchinsonii_medium"]
    ing0_Casitone["Casitone"]
    CultureMech_008924 --> ing0_Casitone
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_008924 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008924 --> ing2_Yeast_extract
    ing3_Cellobiose["Cellobiose"]
    CultureMech_008924 --> ing3_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing3_Cellobiose -.-> CHEBI_17057
    ing4_Distilled_water["Distilled water"]
    CultureMech_008924 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

#	Ingredient	Concentration	Ontology	Notes
1	Casitone	3.0 G_PER_L		DSMZ Medium 67 lists 3 g/L Casitone; this casein digest is retained as an opaque complex component.
2	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	1.36 G_PER_L	CHEBI:86158 calcium chloride dihydrate	TOGO M2337 / DSMZ Medium 160 / DSMZ Medium 67 lists 1.36 g/L CaCl2 x 2 H2O.
3	Yeast extract	1.0 G_PER_L	FOODON:03315426 yeast extract	TOGO M2337 / DSMZ Medium 160 / DSMZ Medium 67 lists 1.0 g/L Yeast extract.
4	Cellobiose C12H22O11 342.297 g/mol	5.0 G_PER_L	CHEBI:17057 cellobiose	DSMZ Medium 160 adds 0.5% w/v filter-sterilized Cellobiose to DSMZ Medium 67, equivalent to 5 g/L.
5	Distilled water H2O 18.015 g/mol	1.0 L	CHEBI:15377 water	DSMZ Medium 67 lists 1000 ml Distilled water.

Preparation

Adjust pH to 7.2.
Add 0.5% filter-sterilized cellobiose to DSMZ Medium 67.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.363974Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2337
2026-01-28T19:48:13.898366Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.314829Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008924
2026-03-17T06:29:55.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.031721+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Cytophaga Hutchinsonii Medium" to "cytophaga_hutchinsonii_medium" for consistent matching
2026-06-05T07:40:36.312737+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00	repair_togo_m2337_score15.py	RESOLVED_TOGO_M2337_SCORE15	TOGO M2337 imports DSMZ Medium 160 as Cytophaga Hutchinsonii Medium. DSMZ Medium 160 adds 0.5% filter-sterilized cellobiose to DSMZ Medium 67. DSMZ Medium 67 lists, per liter, 3 g Casitone, 1.36 g CaCl2 x 2 H2O, 1 g Yeast extract, and 1000 ml Distilled water; the imported M2337 branch is represented without the 15 g agar from DSMZ Medium 67 and with 0.5% w/v Cellobiose converted to 5 g/L. Adjust pH to 7.2. Corrected the imported water g/L artifact, converted cellobiose from 0.5% w/v to 5 g/L, added the DSMZ pH and filter-sterilized cellobiose steps, and marked the liquid cellobiose formula as curated.