nutrient_agar

CultureMech:008928 bacterial COMPLEX SOLID_AGAR
Original name: Nutrient Agar

Overview

External ontology
TOGO:M2340 — Nutrient Agar
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M2340 / DSMZ Medium 1 defines Nutrient Agar using DSMZ Medium 1. DSMZ Medium 1 lists, per liter, 5 g Peptone, 3 g Meat extract, 15 g Agar if necessary, and 1000 ml Distilled water. Adjust pH to 7.0. TOGO M2340 represents the source-recommended Bacillus sporulation addition as 10 mg/L MnSO4 x H2O.

Composition

flowchart LR
    CultureMech_008928["nutrient_agar"]
    ing0_Peptone["Peptone"]
    CultureMech_008928 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_008928 --> ing1_Meat_extract
    ing2_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_008928 --> ing2_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing2_MnSO4_x_H2O -.-> CHEBI_86364
    ing3_Agar__if_necessary["Agar, if necessary"]
    CultureMech_008928 --> ing3_Agar__if_necessary
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_necessary -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_008928 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 DSMZ Medium 1 lists 5 g/L Peptone.
2 Meat extract 3.0 G_PER_L DSMZ Medium 1 lists 3 g/L Meat extract; this generic complex extract is retained without an ontology grounding.
3 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
10.0 MG_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 DSMZ Medium 1 recommends adding 10 mg/L MnSO4 x H2O for Bacillus-strain sporulation.
4 Agar, if necessary 15.0 G_PER_L CHEBI:2509
agar
 DSMZ Medium 1 lists 15 g/L Agar, if necessary.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 DSMZ Medium 1 lists 1000 ml Distilled water.

Preparation

  1. Adjust pH to 7.0.
  2. Add 10 mg/L MnSO4 x H2O for Bacillus-strain sporulation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.368425Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2340
2026-01-28T19:48:20.473050Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.338699Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008928
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.056596+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient Agar" to "nutrient_agar" for consistent matching
2026-06-05T07:40:36.340922+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:48.877546+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00 repair_togo_m2339_m2340_score15.py RESOLVED_TOGO_M2339_M2340_SCORE15 TOGO M2340 / DSMZ Medium 1 defines Nutrient Agar using DSMZ Medium 1. DSMZ Medium 1 lists, per liter, 5 g Peptone, 3 g Meat extract, 15 g Agar if necessary, and 1000 ml Distilled water. Adjust pH to 7.0. TOGO M2340 represents the source-recommended Bacillus sporulation addition as 10 mg/L MnSO4 x H2O. Corrected the imported water and manganese unit artifacts, grounded the disclosed simple and protein-hydrolysate components, kept Meat extract intentionally unmapped, and linked the record to DSMZ Medium 1.
2026-09-12T00:00:00-07:00 repair_nutrient_ncg_nssm_score15.py LINKED_TOGO_M2341_SOURCE_DUPLICATE Linked TOGO M2341 as a source duplicate of the TOGO M2340 Bacillus-sporulation Nutrient Agar formulation.