Overview
- External ontology
- TOGO:M2341 — Nutrient Agar (For sporulation)
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- TOGO M2341 reports the DSMZ Medium 1 sporulation variant for Bacillus strains: 1000 ml distilled water, 5 g peptone, 3 g Meat extract, 15 g agar if necessary, and 10 mg MnSO4 x H2O, adjusted to pH 7.0.
Composition
flowchart LR
CultureMech_008929["nutrient_agar_for_sporulation"]
ing0_Distilled_water["Distilled water"]
CultureMech_008929 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_008929 --> ing1_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing1_MnSO4_x_H2O -.-> CHEBI_86364
ing2_Agar__if_necessary["Agar, if necessary"]
CultureMech_008929 --> ing2_Agar__if_necessary
CHEBI_2509["CHEBI:2509"]
ing2_Agar__if_necessary -.-> CHEBI_2509
ing3_Meat_extract["Meat extract"]
CultureMech_008929 --> ing3_Meat_extract
ing4_Peptone["Peptone"]
CultureMech_008929 --> ing4_Peptone
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M2341 and DSMZ Medium 1 list 1000 ml Distilled water. |
| 2 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
10.0 MG_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
TOGO M2341 and DSMZ Medium 1 recommend adding 10 mg/L MnSO4 x H2O for sporulation of Bacillus strains. |
| 3 | Agar, if necessary | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M2341 and DSMZ Medium 1 list 15.0 g/L Agar if necessary. |
| 4 | Meat extract | 3.0 G_PER_L | TOGO M2341 and DSMZ Medium 1 list 3.0 g/L Meat extract; this generic complex extract is retained without an ontology grounding. | |
| 5 | Peptone | 5.0 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
TOGO M2341 and DSMZ Medium 1 list 5.0 g/L Peptone. |
Preparation
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.369313Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2341 |
| 2026-01-28T19:48:10.747759Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:01.342103Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008929 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.060514+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient Agar (For sporulation)" to "nutrient_agar_for_sporulation" for consistent matching |
| 2026-06-05T07:41:40.120274+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.441853+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-09-12T00:00:00-07:00 | repair_nutrient_ncg_nssm_score15.py | RESOLVED_TOGO_M2341_SCORE15 | TOGO M2341 reports the DSMZ Medium 1 sporulation variant for Bacillus strains: 1000 ml distilled water, 5 g peptone, 3 g Meat extract, 15 g agar if necessary, and 10 mg MnSO4 x H2O, adjusted to pH 7.0. |