Overview
- External ontology
- TOGO:M2350 — 10% Mixotrophic Nitrobacter Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2350 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium756b.pdf
Composition
flowchart LR
CultureMech_008935["10_mixotrophic_nitrobacter_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008935 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008935 --> ing1_Yeast_extract
ing2_Na_pyruvate["Na-pyruvate"]
CultureMech_008935 --> ing2_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Na_pyruvate -.-> CHEBI_50144
ing3_NaNO2["NaNO2"]
CultureMech_008935 --> ing3_NaNO2
CHEBI_78870["CHEBI:78870"]
ing3_NaNO2 -.-> CHEBI_78870
ing4_Peptone["Peptone"]
CultureMech_008935 --> ing4_Peptone
ing5_H3BO3["H3BO3"]
CultureMech_008935 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_008935 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_008935 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_008935 --> ing8_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
ing9_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_008935 --> ing9_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing9_MnSO4_x_H2O -.-> CHEBI_86364
ing10__NH4_6Mo7O24["(NH4)6Mo7O24"]
CultureMech_008935 --> ing10__NH4_6Mo7O24
CHEBI_91249["CHEBI:91249"]
ing10__NH4_6Mo7O24 -.-> CHEBI_91249
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008935 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_NaCl["NaCl"]
CultureMech_008935 --> ing12_NaCl
CHEBI_26710["CHEBI:26710"]
ing12_NaCl -.-> CHEBI_26710
ing13_KH2PO4["KH2PO4"]
CultureMech_008935 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14_CaCO3["CaCO3"]
CultureMech_008935 --> ing14_CaCO3
CHEBI_3311["CHEBI:3311"]
ing14_CaCO3 -.-> CHEBI_3311
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2899.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 899.0, 1000.0, 1000.0] |
| 2 | Yeast extract | 0.15 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.055 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 4 |
NaNO2
NO2.Na 68.995 g/mol
|
2 G_PER_L |
CHEBI:78870
sodium nitrite
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Peptone | 0.15 G_PER_L | ||
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
49.4 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
97.3 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
43.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
25 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
33.8 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)6Mo7O24
2H4N.MoO4 196.014 g/mol
|
37.1 G_PER_L |
CHEBI:91249
ammonium molybdate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CaCO3
CO3.Ca 100.086 g/mol
|
0.07 G_PER_L |
CHEBI:3311
calcium carbonate
|
Role: Protective agent, Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Stock solution
Dose: 100 G_PER_L
See mediadive_6127_Stock_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.383032Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2350 |
| 2026-01-28T19:48:27.755461Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:45.891718+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:16.397995Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:01.417675Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008935 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.139981+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "10% Mixotrophic Nitrobacter Medium" to "10_mixotrophic_nitrobacter_medium" for consistent matching |
| 2026-05-16T09:03:13.194270+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.415961+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.178986+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:26.283152+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.906759+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |