reactivation_with_liquid_medium_1_for_sporulation

CultureMech:008944 bacterial COMPLEX SOLID_AGAR
Original name: Reactivation With Liquid Medium 1 (For sporulation)

Overview

External ontology
TOGO:M2359 — Reactivation With Liquid Medium 1 (For sporulation)
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M2359 / DSMZ Medium 1a defines Reactivation With Liquid Medium 1 using DSMZ Medium 1a. DSMZ Medium 1a lists, per liter, 5 g Peptone, 3 g Meat extract, 15 g Agar if required, and 1000 ml Distilled water. Adjust pH to 7.0. TOGO M2359 represents the source-recommended Bacillus sporulation addition as 10 mg/L MnSO4 x H2O.

Composition

flowchart LR
    CultureMech_008944["reactivation_with_liquid_medium_1_for_sporulation"]
    ing0_Peptone["Peptone"]
    CultureMech_008944 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_008944 --> ing1_Meat_extract
    ing2_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_008944 --> ing2_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing2_MnSO4_x_H2O -.-> CHEBI_86364
    ing3_Agar__if_required["Agar, if required"]
    CultureMech_008944 --> ing3_Agar__if_required
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_required -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_008944 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 DSMZ Medium 1a lists 5 g/L Peptone.
2 Meat extract 3.0 G_PER_L DSMZ Medium 1a lists 3 g/L Meat extract; this generic complex extract is retained without an ontology grounding.
3 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
10.0 MG_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 DSMZ Medium 1a recommends adding 10 mg/L MnSO4 x H2O for Bacillus-strain sporulation.
4 Agar, if required 15.0 G_PER_L CHEBI:2509
agar
 DSMZ Medium 1a lists 15 g/L Agar, if required.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 DSMZ Medium 1a lists 1000 ml Distilled water.

Preparation

  1. Adjust pH to 7.0.
  2. Add 10 mg/L MnSO4 x H2O for Bacillus-strain sporulation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.394550Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2359
2026-01-28T19:48:15.136648Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.459550Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008944
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.184545+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Reactivation With Liquid Medium 1 (For sporulation)" to "reactivation_with_liquid_medium_1_for_sporulation" for consistent matching
2026-06-05T07:41:44.075814+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.154715+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-09-11T00:00:00-07:00 repair_togo_m2358_m2359_score15.py RESOLVED_TOGO_M2358_M2359_SCORE15 TOGO M2359 / DSMZ Medium 1a defines Reactivation With Liquid Medium 1 using DSMZ Medium 1a. DSMZ Medium 1a lists, per liter, 5 g Peptone, 3 g Meat extract, 15 g Agar if required, and 1000 ml Distilled water. Adjust pH to 7.0. TOGO M2359 represents the source-recommended Bacillus sporulation addition as 10 mg/L MnSO4 x H2O. Corrected the imported water and manganese unit artifacts, grounded the disclosed simple and protein-hydrolysate components, kept Meat extract intentionally unmapped, and linked the record to DSMZ Medium 1a.