Overview
- External ontology
- TOGO:M2369 — Halobacteria Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2369 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium372.pdf
Composition
flowchart LR
CultureMech_008953["halobacteria_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008953 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008953 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008953 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008953 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_008953 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_008953 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_Na_glutamate["Na-glutamate"]
CultureMech_008953 --> ing6_Na_glutamate
CHEBI_64220["CHEBI:64220"]
ing6_Na_glutamate -.-> CHEBI_64220
ing7_Na3_citrate["Na3-citrate"]
CultureMech_008953 --> ing7_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing7_Na3_citrate -.-> CHEBI_53258
ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_008953 --> ing8_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
ing9_Agar["Agar"]
CultureMech_008953 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Casamino_acids["Casamino acids"]
CultureMech_008953 --> ing10_Casamino_acids
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
Na-glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Simple component, Organic compound, Defined component |
| 8 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 9 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 11 | Casamino acids | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.409139Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2369 |
| 2026-01-28T19:48:19.043088Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:01.539284Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008953 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.263568+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halobacteria Medium" to "halobacteria_medium" for consistent matching |
| 2026-06-05T07:40:36.527582+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.223706+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:26.379364+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |