Overview
- External ontology
- TOGO:M2371 — Nitrosolobus medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2371 Original URL: https://www.atcc.org/~/media/55C41057CA124E1381E3BDCFC87EC334.ashx
Composition
flowchart LR
CultureMech_008956["nitrosolobus_medium"]
ing0_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008956 --> ing0_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_7H2O -.-> CHEBI_31795
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008956 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_K2HPO4["K2HPO4"]
CultureMech_008956 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Na2MoO4_2H2O["Na2MoO4.2H2O"]
CultureMech_008956 --> ing3_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing3_Na2MoO4_2H2O -.-> CHEBI_75213
ing4_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008956 --> ing4_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_4H2O -.-> CHEBI_86368
ing5_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008956 --> ing5_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing5_CoCl2_6H2O -.-> CHEBI_35696
ing6_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008956 --> ing6_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_7H2O -.-> CHEBI_32312
ing7_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_008956 --> ing7_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing7_Ammonium_sulfate -.-> CHEBI_62946
ing8_DI_water["DI water"]
CultureMech_008956 --> ing8_DI_water
ing9_Phenol_red_0_5___see_below_["Phenol red 0.5% (see below)"]
CultureMech_008956 --> ing9_Phenol_red_0_5___see_below_
ing10_HCl_conc_["HCl(conc)"]
CultureMech_008956 --> ing10_HCl_conc_
ing11_EDTA__sodium_salt["EDTA, sodium salt"]
CultureMech_008956 --> ing11_EDTA__sodium_salt
ing12_6N_NaOH["6N NaOH"]
CultureMech_008956 --> ing12_6N_NaOH
ing13_Phenol_Red["Phenol Red"]
CultureMech_008956 --> ing13_Phenol_Red
CHEBI_31991["CHEBI:31991"]
ing13_Phenol_Red -.-> CHEBI_31991
ing14_K2CO3["K2CO3"]
CultureMech_008956 --> ing14_K2CO3
CHEBI_131526["CHEBI:131526"]
ing14_K2CO3 -.-> CHEBI_131526
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
380 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
20 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
87 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Na2MoO4.2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
100 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
200 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
100 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
1.32 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 | DI water | 300.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 1.0, 100.0, 99.0, 100.0] | |
| 10 | Phenol red 0.5% (see below) | 0.25 G_PER_L | ||
| 11 | HCl(conc) | 0.3 G_PER_L | Role: Solvating media | |
| 12 | EDTA, sodium salt | 0.2 G_PER_L | Properties: Defined component, Simple component | |
| 13 | 6N NaOH | 1 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 14 |
Phenol Red
C19H14O5S 354.383 g/mol
|
0.5 G_PER_L |
CHEBI:31991
phenol red
|
Role: pH indicator; Properties: Organic compound, Defined component, Simple component |
| 15 |
K2CO3
CO3.2K 138.204 g/mol
|
6.91 G_PER_L |
CHEBI:131526
potassium carbonate
|
Properties: Defined component, Simple component |
Solutions
Chelated Iron Solution
Dose: 1 G_PER_L
See mediadive_4790_Chelated_iron_solution.yaml for composition
0.5 M K2CO3 Solution
Dose: variable VARIABLE
Ferric (III) ammonium citrate*
Dose: 0.1 G_PER_L
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.411761Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2371 |
| 2026-01-28T19:48:23.631159Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 0.5 M K2CO3 Solution |
| 2026-03-09T08:04:39.317282+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:09.157430Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:01.566896Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008956 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.291321+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nitrosolobus medium" to "nitrosolobus_medium" for consistent matching |
| 2026-05-16T09:04:27.693613+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:39.822716+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:28.242775+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.601148+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |