Overview
- External ontology
- TOGO:M2376 — Artificial deep lake medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2376 Original URL: https://www.atcc.org/~/media/7D0A842AFDC848C393970526DC9F9390.ashx
Composition
flowchart LR
CultureMech_008960["artificial_deep_lake_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008960 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_008960 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008960 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008960 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2___2H2O["CaCl2 · 2H2O"]
CultureMech_008960 --> ing4_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2___2H2O -.-> CHEBI_86158
ing5_MgCl2___6H2O["MgCl2 · 6H2O"]
CultureMech_008960 --> ing5_MgCl2___6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2___6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_008960 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Sodium_succinate["Sodium succinate"]
CultureMech_008960 --> ing7_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing7_Sodium_succinate -.-> CHEBI_63675
ing8_Agar__Noble__BD_214230_["Agar, Noble (BD 214230)"]
CultureMech_008960 --> ing8_Agar__Noble__BD_214230_
ing9_Biotin["Biotin"]
CultureMech_008960 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Thiamine___HCl["Thiamine . HCl"]
CultureMech_008960 --> ing10_Thiamine___HCl
ing11_Cyanocobalamin["Cyanocobalamin"]
CultureMech_008960 --> ing11_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing11_Cyanocobalamin -.-> CHEBI_17439
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
991.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 990.0, 1.0] |
| 2 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
7.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
CaCl2 · 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
MgCl2 · 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
7.4 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
10 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Organic compound, Simple component, Defined component |
| 9 | Agar, Noble (BD 214230) | 15 G_PER_L | Role: Solidifying component; Properties: Defined component | |
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 11 | Thiamine . HCl | 0.1 G_PER_L | Role: Vitamin; Properties: Simple component, Organic compound, Defined component | |
| 12 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
0.1 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin |
Solutions
Vitamin Solution
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.419028Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2376 |
| 2026-01-28T19:48:07.978286Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:12.844565+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:39.750946Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.594223Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008960 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.321194+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Artificial deep lake medium" to "artificial_deep_lake_medium" for consistent matching |
| 2026-05-16T09:03:24.881949+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:49.712402+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:36.749987+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |