halorhabdus_utahensis_medium

CultureMech:008961 archaea COMPLEX LIQUID
Original name: Halorhabdus Utahensis Medium

Overview

External ontology
TOGO:M2377 — Halorhabdus Utahensis Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M2377 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium927.pdf

Composition

flowchart LR
    CultureMech_008961["halorhabdus_utahensis_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008961 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008961 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_008961 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_008961 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KCl["KCl"]
    CultureMech_008961 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaBr["NaBr"]
    CultureMech_008961 --> ing5_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing5_NaBr -.-> CHEBI_63004
    ing6_Tris_HCl["Tris-HCl"]
    CultureMech_008961 --> ing6_Tris_HCl
    ing7_Glucose["Glucose"]
    CultureMech_008961 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Yeast_extract__Difco_["Yeast extract (Difco)"]
    CultureMech_008961 --> ing8_Yeast_extract__Difco_
    ing9_NaOH["NaOH"]
    CultureMech_008961 --> ing9_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing9_NaOH -.-> CHEBI_32145
    ing10_KH2PO4["KH2PO4"]
    CultureMech_008961 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_008961 --> ing11_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing11_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_008961 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_008961 --> ing13_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing13_FeCl2_x_4_H2O -.-> CHEBI_86249

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000.0 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 4 identical duplicates: 1000.0, 1000.0, 1000.0, 1000.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 NaCl
Cl.Na 58.443 g/mol
270 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 NH4Cl
Cl.H4N 53.492 g/mol
2 G_PER_L CHEBI:31206
ammonium chloride
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
5 KCl
Cl.K 74.551 g/mol
5 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 NaBr
Br.Na 102.894 g/mol
0.1 G_PER_L CHEBI:63004
sodium bromide
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 Tris-HCl 12 G_PER_L Role: Buffer; Properties: Defined component, Complex component, Solution
8 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Organic compound, Defined component, Simple component
9 Yeast extract (Difco) 1 G_PER_L Properties: Undefined component
10 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
Properties: Defined component, Inorganic compound, Solution
11 KH2PO4
H2O4P.K 136.084 g/mol
50 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
12 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
100 G_PER_L CHEBI:91243
calcium chloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
20 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
20 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

phosphate solution

Dose: 2.5 G_PER_L

See mediadive_4669_Phosphate_solution.yaml for composition

calcium chloride solution

Dose: 0.5 G_PER_L

Fe/Mn solution

Dose: 0.25 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.420923Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2377
2026-01-28T19:48:23.604657Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-02-06T05:10:32.244430Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-09T08:04:39.270077+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:09:09.110736Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:20:01.603563Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008961
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.331008+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Halorhabdus Utahensis Medium" to "halorhabdus_utahensis_medium" for consistent matching
2026-05-16T09:03:13.331164+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:36.577076+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:26.428537+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:48.953938+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:37:26.995820+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.