Overview
- External ontology
- TOGO:M2400 — Middlebrook 7H9 liquid medium supplemented with 10% albumin, dextrose, and catalase
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2400 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. False LB constituent expansion is withdrawn. Source-specific amounts and preparation boundaries supersede flattened concentrations. Existing chemical annotations and earlier history are preserved, not recertified; no organism-growth association is added. Source preparation context (main preparation): Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Source preparation context (main preparation): Cool to 45 °C or below and aseptically add 1 vial ofMiddlebrook ADC Growth Supplement. Mix well before dispensing.
Composition
flowchart LR
CultureMech_008983["middlebrook_7h9_liquid_medium_supplemented_with_10_albumin_dextrose..."]
ing0_Distilled_water["Distilled water"]
CultureMech_008983 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glycerol["Glycerol"]
CultureMech_008983 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
0.45 L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 450.0, 50.0] Source amount: 450 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 450 ml = 0.45 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it. |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
0.002 L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 2 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 2 ml = 0.002 L. |
Solutions
Middlebrook 7H9 Broth Base
| Ingredient | Concentration | Notes |
|---|---|---|
| Monopotassium Phosphate | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Biotin | Role: Vitamin; Properties: Organic compound, Defined component, Simple component Source amount: 0.0005 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Ammonium Sulfate | Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.5 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Calcium Chloride | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.0005 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Disodium Phosphate | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source amount: 2.5 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Magnesium Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.05 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Copper Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.001 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Sodium Citrate | Role: Buffer; Properties: Organic compound, Defined component, Simple component Source amount: 0.1 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Ferric Ammonium Citrate | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component Source amount: 0.04 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| L-Glutamic Acid | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component Source amount: 0.5 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Pyridoxine | Role: Vitamin; Properties: Organic compound, Defined component, Simple component Source amount: 0.001 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Zinc Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.001 g; scope: Middlebrook 7H9 Broth Base formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Source amount: 2.35 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. Inline commercial-base formula detail, not extra simultaneous ingredients. Its table masses have no verified volume/dilution basis.
Source preparation context (Middlebrook 7H9 Broth Base): Final pH 6.6 +/- 0.2 at 25°C
Middlebrook ADC Growth Supplement
Dose: 0.05 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.05 L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 450.0, 50.0] Source amount: 50 ml; scope: Middlebrook ADC Growth Supplement stock preparation. Volume-only input/dose, not a final-volume marker; 50 ml = 0.05 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it. |
| Dextrose | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 1 g; scope: Middlebrook ADC Growth Supplement stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Bovine albumin fraction | Source amount: 2.5 g; scope: Middlebrook ADC Growth Supplement stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Catalase | Properties: Defined component, Simple component Source amount: 0.0015 g; scope: Middlebrook ADC Growth Supplement stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Source amount: 50 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 50 ml = 0.05 L. Inline enrichment composition is separate from its dose and from finished-medium concentrations.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:56.459711Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2400 |
| 2026-01-28T19:48:16.732855Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:56.388004Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.795209Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008983 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.538344+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middlebrook 7H9 liquid medium supplemented with 10% albumin, dextrose, and catalase" to "middlebrook_7h9_liquid_medium_supplemented_with_10_albumin_dextrose_and_catalase" for consistent matching |
| 2026-06-05T07:41:34.013968+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.273379+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-09T00:43:54.768901+00:00 | codex | RESTORED_MIDDLEBROOK_INGREDIENT_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore albumin and separate basal inputs, commercial-base detail and ADC/OADC stock amounts/doses. Correct volume/mass confusion; retain unsupported mass/percent bases and source-version conflicts explicitly. Preserve IDs and all prior history. |