Overview
- External ontology
- TOGO:M2403 — MADCTw medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2403 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. False LB constituent expansion is withdrawn. Source-specific amounts and preparation boundaries supersede flattened concentrations. Existing chemical annotations and earlier history are preserved, not recertified; no organism-growth association is added. Source preparation context (main preparation): Middlebrook 7H9 medium (Difco) containing 10% (v/v) albumin‐dextrose‐catalase (ADC, Difco) enrichment, 0.2% glycerol and 0.05% Tween 80 (MADCTw)
Composition
flowchart LR
CultureMech_008986["madctw_medium"]
ing0_Tween_80["Tween 80"]
CultureMech_008986 --> ing0_Tween_80
CHEBI_53426["CHEBI:53426"]
ing0_Tween_80 -.-> CHEBI_53426
ing1_Glycerol["Glycerol"]
CultureMech_008986 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_DI_Water["DI Water"]
CultureMech_008986 --> ing2_DI_Water
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Defined component, Simple component Source amount: 0.05 %; scope: main preparation input. Percentage basis is unspecified; the earlier w/v or g/L assertion is withdrawn, not replaced with an assumed basis. | |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 0.2 %; scope: main preparation input. Percentage basis is unspecified; the earlier w/v or g/L assertion is withdrawn, not replaced with an assumed basis. | |
| 3 | DI Water | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [WARNING: 2 duplicates with conflicting units] [MANUAL REVIEW REQUIRED: PERCENT_W_V incompatible with absolute units] Source amount: 90 %; scope: main preparation input. Percentage basis is unspecified; the earlier w/v or g/L assertion is withdrawn, not replaced with an assumed basis. |
Solutions
Middlebrook 7H9 broth
| Ingredient | Concentration | Notes |
|---|---|---|
| Monopotassium Phosphate | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Biotin | Role: Vitamin; Properties: Organic compound, Defined component, Simple component Source amount: 0.5 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Ammonium Sulfate | Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.5 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Calcium Chloride | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.5 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Disodium Phosphate | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source amount: 2.5 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Magnesium Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.05 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Copper Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Sodium Citrate | Role: Buffer; Properties: Organic compound, Defined component, Simple component Source amount: 0.1 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Ferric Ammonium Citrate | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component Source amount: 0.04 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| L-Glutamic Acid | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component Source amount: 0.5 g; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Pyridoxine | Role: Vitamin; Properties: Organic compound, Defined component, Simple component Source amount: 1 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Zinc Sulfate | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 mg; scope: Middlebrook 7H9 broth formula-detail table. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Source amount: 4.7 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. Inline commercial-base formula detail, not extra simultaneous ingredients. Its table masses have no verified volume/dilution basis.
ADC Enrichment
Dose: 10 PERCENT_V_V
| Ingredient | Concentration | Notes |
|---|---|---|
| Dextrose | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 2 g; scope: ADC Enrichment stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Bovine Serum Albumin, Frac V | Source amount: 5 g; scope: ADC Enrichment stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| DI Water | 0.1 L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [WARNING: 2 duplicates with conflicting units] [MANUAL REVIEW REQUIRED: PERCENT_W_V incompatible with absolute units] Source amount: 100 ml; scope: ADC Enrichment stock preparation. Volume-only input/dose, not a final-volume marker; 100 ml = 0.1 L. |
| Catalase (beef) | Properties: Defined component, Simple component Source amount: 3 mg; scope: ADC Enrichment stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Source amount: 10 % (v/v); scope: main preparation input. The percentage basis is explicitly volume/volume. Inline enrichment composition is separate from its dose and from finished-medium concentrations.
Source preparation context (ADC Enrichment): Filter Sterilize.
Source preparation context (ADC Enrichment): Store any unused portion with lot information and Media Number in 2-8ºC.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.467371Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2403 |
| 2026-01-28T19:48:22.695141Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:07.557575Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s); WARNING: Duplicate 'DI Water' with different units: {'G_PER_L', 'PERCENT_W_V'} |
| 2026-03-13T04:23:43.780684Z | unit-conflict-resolver-v1.0 | Resolved unit conflicts | Flagged DI Water for manual review (PERCENT_W_V conflict) |
| 2026-03-13T06:20:01.827936Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008986 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.571992+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MADCTw medium" to "madctw_medium" for consistent matching |
| 2026-06-05T07:41:12.494474+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.617921+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-09T00:43:54.768901+00:00 | codex | RESTORED_MIDDLEBROOK_INGREDIENT_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore albumin and separate basal inputs, commercial-base detail and ADC/OADC stock amounts/doses. Correct volume/mass confusion; retain unsupported mass/percent bases and source-version conflicts explicitly. Preserve IDs and all prior history. |