bsm_9_1_mixture_v_v_of_bsm_plus_lb

CultureMech:008988 bacterial COMPLEX SOLID_AGAR
Original name: BSM+ (9:1 mixture v/v of BSM plus LB)

Overview

External ontology
TOGO:M2405 — BSM+ (9:1 mixture v/v of BSM plus LB)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2405 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_008988["bsm_9_1_mixture_v_v_of_bsm_plus_lb"]
    ing0_Tryptone["Tryptone"]
    CultureMech_008988 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008988 --> ing1_Yeast_extract
    ing2_Sodium_chloride["Sodium chloride"]
    CultureMech_008988 --> ing2_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_chloride -.-> CHEBI_26710
    ing3_Basal_salts_medium__BSM_["Basal salts medium (BSM)"]
    CultureMech_008988 --> ing3_Basal_salts_medium__BSM_
    ing4_Distilled_water["Distilled water"]
    CultureMech_008988 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_MgSO4___7H2O["MgSO4 . 7H2O"]
    CultureMech_008988 --> ing5_MgSO4___7H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4___7H2O -.-> CHEBI_31795
    ing6_NaCl["NaCl"]
    CultureMech_008988 --> ing6_NaCl
    ing6_NaCl -.-> CHEBI_26710
    ing7_KH2PO4["KH2PO4"]
    CultureMech_008988 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_FeSO4___7H2O["FeSO4 . 7H2O"]
    CultureMech_008988 --> ing8_FeSO4___7H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4___7H2O -.-> CHEBI_75836
    ing9__NH4_SO4["(NH4)SO4"]
    CultureMech_008988 --> ing9__NH4_SO4
    ing10_Na2HPO4["Na2HPO4"]
    CultureMech_008988 --> ing10_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing10_Na2HPO4 -.-> CHEBI_34683
    ing11_CaCl2___7H2O["CaCl2 . 7H2O"]
    CultureMech_008988 --> ing11_CaCl2___7H2O
    CHEBI_3312["CHEBI:3312"]
    ing11_CaCl2___7H2O -.-> CHEBI_3312
    ing12_Agar["Agar"]
    CultureMech_008988 --> ing12_Agar
    CHEBI_2509["CHEBI:2509"]
    ing12_Agar -.-> CHEBI_2509

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
10.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
2 Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
5.0 G_PER_L FOODON:03315426
yeast extract
 Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
3 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
10.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product
4 Basal salts medium (BSM) 90 G_PER_L
5 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
6 MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 NaCl
Cl.Na 58.443 g/mol
0.5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 KH2PO4
H2O4P.K 136.084 g/mol
1.36 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
9 FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
0.28 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 (NH4)SO4 2.38 G_PER_L Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
11 Na2HPO4
HO4P.2Na 141.958 g/mol
1.42 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
12 CaCl2 . 7H2O
Ca.2Cl 110.984 g/mol
10.69 G_PER_L CHEBI:3312
calcium dichloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
13 Agar 1.5 PERCENT_W_V CHEBI:2509
agar
 Role: Solidifying component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.472440Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2405
2026-01-28T19:48:37.572303Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.847128Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008988
2026-03-17T06:30:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.591805+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BSM+ (9:1 mixture v/v of BSM plus LB)" to "bsm_9_1_mixture_v_v_of_bsm_plus_lb" for consistent matching
2026-06-05T07:40:51.690336+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:38.090855+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:53.993703+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.