Overview
- External ontology
- TOGO:M2407 — Middlebrook 7H11 agar (Difco)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2407 Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. False LB constituent expansion is withdrawn. Source-specific amounts and preparation boundaries supersede flattened concentrations. Existing chemical annotations and earlier history are preserved, not recertified; no organism-growth association is added.
Composition
flowchart LR
CultureMech_008990["middlebrook_7h11_agar_difco"]
ing0_Distilled_water["Distilled water"]
CultureMech_008990 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Monopotassium_Phosphate["Monopotassium Phosphate"]
CultureMech_008990 --> ing1_Monopotassium_Phosphate
CHEBI_63036["CHEBI:63036"]
ing1_Monopotassium_Phosphate -.-> CHEBI_63036
ing2_Biotin["Biotin"]
CultureMech_008990 --> ing2_Biotin
CHEBI_15956["CHEBI:15956"]
ing2_Biotin -.-> CHEBI_15956
ing3_Ammonium_Sulfate["Ammonium Sulfate"]
CultureMech_008990 --> ing3_Ammonium_Sulfate
CHEBI_62946["CHEBI:62946"]
ing3_Ammonium_Sulfate -.-> CHEBI_62946
ing4_Calcium_Chloride["Calcium Chloride"]
CultureMech_008990 --> ing4_Calcium_Chloride
CHEBI_86158["CHEBI:86158"]
ing4_Calcium_Chloride -.-> CHEBI_86158
ing5_Disodium_Phosphate["Disodium Phosphate"]
CultureMech_008990 --> ing5_Disodium_Phosphate
CHEBI_34683["CHEBI:34683"]
ing5_Disodium_Phosphate -.-> CHEBI_34683
ing6_Glycerol["Glycerol"]
CultureMech_008990 --> ing6_Glycerol
CHEBI_17754["CHEBI:17754"]
ing6_Glycerol -.-> CHEBI_17754
ing7_Monosodium_Glutamate["Monosodium Glutamate"]
CultureMech_008990 --> ing7_Monosodium_Glutamate
CHEBI_64220["CHEBI:64220"]
ing7_Monosodium_Glutamate -.-> CHEBI_64220
ing8_Magnesium_Sulfate["Magnesium Sulfate"]
CultureMech_008990 --> ing8_Magnesium_Sulfate
CHEBI_32599["CHEBI:32599"]
ing8_Magnesium_Sulfate -.-> CHEBI_32599
ing9_Copper_Sulfate["Copper Sulfate"]
CultureMech_008990 --> ing9_Copper_Sulfate
CHEBI_23414["CHEBI:23414"]
ing9_Copper_Sulfate -.-> CHEBI_23414
ing10_Sodium_Citrate["Sodium Citrate"]
CultureMech_008990 --> ing10_Sodium_Citrate
CHEBI_53258["CHEBI:53258"]
ing10_Sodium_Citrate -.-> CHEBI_53258
ing11_Ferric_Ammonium_Citrate["Ferric Ammonium Citrate"]
CultureMech_008990 --> ing11_Ferric_Ammonium_Citrate
CHEBI_31604["CHEBI:31604"]
ing11_Ferric_Ammonium_Citrate -.-> CHEBI_31604
ing12_Pyridoxine["Pyridoxine"]
CultureMech_008990 --> ing12_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing12_Pyridoxine -.-> CHEBI_16709
ing13_Zinc_Sulfate["Zinc Sulfate"]
CultureMech_008990 --> ing13_Zinc_Sulfate
CHEBI_35176["CHEBI:35176"]
ing13_Zinc_Sulfate -.-> CHEBI_35176
ing14_Malachite_Green["Malachite Green"]
CultureMech_008990 --> ing14_Malachite_Green
CHEBI_72449["CHEBI:72449"]
ing14_Malachite_Green -.-> CHEBI_72449
ing15_Agar["Agar"]
CultureMech_008990 --> ing15_Agar
CHEBI_2509["CHEBI:2509"]
ing15_Agar -.-> CHEBI_2509
ing16_Pancreatic_Digest_of_Casein["Pancreatic Digest of Casein"]
CultureMech_008990 --> ing16_Pancreatic_Digest_of_Casein
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
0.9 L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 900.0, 1.0] Source amount: 900 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 900 ml = 0.9 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it. |
| 2 |
Monopotassium Phosphate
H2O4P.K 136.084 g/mol
|
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source amount: 1.5 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 3 |
Biotin
C10H16N2O3S 244.316 g/mol
|
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component Source amount: 0.5 mg; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 4 |
Ammonium Sulfate
2H4N.O4S 132.141 g/mol
|
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.5 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 5 |
Calcium Chloride
Ca.2Cl.2H2O 147.014 g/mol
|
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.5 mg; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 6 |
Disodium Phosphate
HO4P.2Na 141.958 g/mol
|
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source amount: 1.5 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 7 |
Glycerol
C3H8O3 92.094 g/mol
|
0.005 L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 5 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 5 ml = 0.005 L. |
| 8 |
Monosodium Glutamate
C5H8NO4.Na 169.112 g/mol
|
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component Source amount: 0.5 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 9 |
Magnesium Sulfate
Mg.O4S 120.368 g/mol
|
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 0.05 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 10 |
Copper Sulfate
Cu.O4S 159.609 g/mol
|
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 mg; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 11 |
Sodium Citrate
C6H5O7.3Na 258.069 g/mol
|
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component Source amount: 0.4 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 12 | Ferric Ammonium Citrate |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component Source amount: 0.04 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 13 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
CHEBI:16709
pyridoxine
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component Source amount: 1 mg; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 14 |
Zinc Sulfate
O4S.Zn 161.453 g/mol
|
CHEBI:35176
zinc sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 1 mg; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 15 |
Malachite Green
C23H25N2.Cl 364.92 g/mol
|
CHEBI:72449
malachite green
|
Role: pH indicator, Selective agent; Properties: Organic compound, Defined component, Simple component Source amount: 0.25 mg; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 16 | Agar |
CHEBI:2509
agar
|
Role: Solidifying component Source amount: 13.5 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| 17 | Pancreatic Digest of Casein | Properties: Undefined component Source amount: 1 g; scope: main preparation input. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Solutions
OADC Enrichment (Difco)
Dose: 0.1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 1 L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 900.0, 1.0] Source amount: 1 L; scope: OADC Enrichment (Difco) stock preparation. Volume-only input/dose, not a final-volume marker; 1 L = 1 L. Earlier merged-quantity text is retained only as historical provenance; the separated source stages supersede it. |
| Sodium chloride | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source amount: 8.5 g; scope: OADC Enrichment (Difco) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Dextrose | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 20 g; scope: OADC Enrichment (Difco) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Bovine Albumin (Fraction V) | Source amount: 50 g; scope: OADC Enrichment (Difco) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. | |
| Oleic Acid | 0.0006 L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source amount: 0.6 ml; scope: OADC Enrichment (Difco) stock preparation. Volume-only input/dose, not a final-volume marker; 0.6 ml = 0.0006 L. |
| Catalase | Properties: Defined component, Simple component Source amount: 0.03 g; scope: OADC Enrichment (Difco) stock preparation. This table does not give an explicit final-volume denominator. Preserve the exact mass here; no g/L conversion is asserted. The schema has no mass-only concentration unit. |
Source amount: 100 ml; scope: main preparation input. Volume-only input/dose, not a final-volume marker; 100 ml = 0.1 L. Inline enrichment composition is separate from its dose and from finished-medium concentrations.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:56.474494Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2407 |
| 2026-01-28T19:48:20.452410Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:03.418691Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.859315Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008990 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.604104+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middlebrook 7H11 agar (Difco)" to "middlebrook_7h11_agar_difco" for consistent matching |
| 2026-06-05T07:41:33.947309+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.213226+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-10-09T00:43:54.768901+00:00 | codex | RESTORED_MIDDLEBROOK_INGREDIENT_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore albumin and separate basal inputs, commercial-base detail and ADC/OADC stock amounts/doses. Correct volume/mass confusion; retain unsupported mass/percent bases and source-version conflicts explicitly. Preserve IDs and all prior history. |