Overview
- External ontology
- TOGO:M2420 — Alkaline Nutrient Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2420 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium31.pdf
Composition
flowchart LR
CultureMech_009003["alkaline_nutrient_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_009003 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Agar__if_necessary["Agar, if necessary"]
CultureMech_009003 --> ing1_Agar__if_necessary
ing2_Meat_extract["Meat extract"]
CultureMech_009003 --> ing2_Meat_extract
ing3_Peptone["Peptone"]
CultureMech_009003 --> ing3_Peptone
ing4_NaHCO3["NaHCO3"]
CultureMech_009003 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Na2CO3_anhydrous["Na2CO3 anhydrous"]
CultureMech_009003 --> ing5_Na2CO3_anhydrous
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3_anhydrous -.-> CHEBI_29377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1100.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 1000.0, 100.0] |
| 2 | Agar, if necessary | 15 G_PER_L | Role: Solidifying component | |
| 3 | Meat extract | 3 G_PER_L | Properties: Undefined component | |
| 4 | Peptone | 5 G_PER_L | ||
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
Na2CO3 anhydrous
CO3.2Na 105.988 g/mol
|
5.3 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
Solutions
Na-sesquicarbonate solution
Dose: 100 G_PER_L
See mediadive_1174_Na-sesquicarbonate_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.488331Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2420 |
| 2026-01-28T19:48:29.874127Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:49.673008+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:20.835885Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.915042Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009003 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.664923+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Nutrient Agar" to "alkaline_nutrient_agar" for consistent matching |
| 2026-05-16T09:03:13.471507+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.733475+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |