Overview
- External ontology
- TOGO:M2439 — MRS Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2439 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium11.pdf
Composition
flowchart LR
CultureMech_009018["mrs_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009018 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009018 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009018 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_009018 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Na_acetate["Na-acetate"]
CultureMech_009018 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5_Tween_80["Tween 80"]
CultureMech_009018 --> ing5_Tween_80
CHEBI_53426["CHEBI:53426"]
ing5_Tween_80 -.-> CHEBI_53426
ing6_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_009018 --> ing6_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing6_MnSO4_x_H2O -.-> CHEBI_86364
ing7__NH4_3_citrate["(NH4)3 citrate"]
CultureMech_009018 --> ing7__NH4_3_citrate
CHEBI_63037["CHEBI:63037"]
ing7__NH4_3_citrate -.-> CHEBI_63037
ing8_Glucose["Glucose"]
CultureMech_009018 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Meat_extract["Meat extract"]
CultureMech_009018 --> ing9_Meat_extract
ing10_Casein_peptone__tryptic_digest["Casein peptone, tryptic digest"]
CultureMech_009018 --> ing10_Casein_peptone__tryptic_digest
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 6 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Simple component, Defined component |
| 7 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.05 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
(NH4)3 citrate
C6H5O7.3H4N 243.216 g/mol
|
2 G_PER_L |
CHEBI:63037
triammonium citrate
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 10 | Meat extract | 10 G_PER_L | Properties: Undefined component | |
| 11 | Casein peptone, tryptic digest | 10 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.501588Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2439 |
| 2026-01-28T19:48:26.037837Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:01.970166Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009018 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.724406+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRS Medium" to "mrs_medium" for consistent matching |
| 2026-06-05T07:40:36.740222+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.301683+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:26.584169+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.006336+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |