modified_337a_agar_medium

CultureMech:009024 bacterial COMPLEX SOLID_AGAR
Original name: Modified 337a Agar Medium

Overview

External ontology
TOGO:M2445 — Modified 337a Agar Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2445 Original URL: https://www.atcc.org/~/media/E4D6EB3B1C80408996E0DD53A0D258A1.ashx

Composition

flowchart LR
    CultureMech_009024["modified_337a_agar_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009024 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_KH2PO4["KH2PO4"]
    CultureMech_009024 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009024 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_Na2HPO4["Na2HPO4"]
    CultureMech_009024 --> ing3_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4 -.-> CHEBI_34683
    ing4_DI_Water["DI Water"]
    CultureMech_009024 --> ing4_DI_Water
    ing5_Agar["Agar"]
    CultureMech_009024 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Vitamin_B12__0_1_mg_ml__filter_sterilized_["Vitamin B12 (0.1 mg/ml, filter sterilized)"]
    CultureMech_009024 --> ing6_Vitamin_B12__0_1_mg_ml__filter_sterilized_
    ing7_Methanol_filter_sterilized_["Methanol(filter sterilized)"]
    CultureMech_009024 --> ing7_Methanol_filter_sterilized_
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_009024 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_009024 --> ing9_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_009024 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_009024 --> ing11_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing11_MnSO4_x_4_H2O -.-> CHEBI_86358

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
2 KH2PO4
H2O4P.K 136.084 g/mol
1.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
4 Na2HPO4
HO4P.2Na 141.958 g/mol
1.13 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 DI Water 1550.0 G_PER_L Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 3 duplicates: 1000.0, 500.0, 50.0]
6 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
7 Vitamin B12 (0.1 mg/ml, filter sterilized) 1 G_PER_L Role: Vitamin; Properties: Defined component, Organic compound, Simple component
8 Methanol(filter sterilized) 3 G_PER_L Role: Solvating media; Properties: Organic compound, Solution, Defined component
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
30.9 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
10 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
10 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
20 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
12 MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
8.8 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component

Solutions

Trace Element Solution

Dose: 5 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.510702Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2445
2026-01-28T19:48:34.341072Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:57.008479+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:28.583279Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:02.021935Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009024
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.779659+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified 337a Agar Medium" to "modified_337a_agar_medium" for consistent matching
2026-05-16T09:04:24.944107+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:36.058821+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:25.598221+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:08.471629+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.