lb_agar_miller

CultureMech:009026 bacterial COMPLEX SOLID_AGAR
Original name: LB Agar, Miller

Overview

External ontology
TOGO:M2447 — LB Agar, Miller
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.2}
Notes
TOGO M2447 imports the scratch LB Agar, Miller formulation from ATCC Medium 1065. ATCC lists 10.0 g Tryptone, 5.0 g Yeast Extract, 10 g Sodium Chloride, and 15.0 g Agar; TOGO M2447 retains the one-liter DI Water row. Final pH is 7.0 +/- 0.2.

Composition

flowchart LR
    CultureMech_009026["lb_agar_miller"]
    ing0_Tryptone["Tryptone"]
    CultureMech_009026 --> ing0_Tryptone
    ing1_Yeast_Extract["Yeast Extract"]
    CultureMech_009026 --> ing1_Yeast_Extract
    ing2_Sodium_Chloride["Sodium Chloride"]
    CultureMech_009026 --> ing2_Sodium_Chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_Chloride -.-> CHEBI_26710
    ing3_Agar["Agar"]
    CultureMech_009026 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_DI_Water["DI Water"]
    CultureMech_009026 --> ing4_DI_Water
    CHEBI_15377["CHEBI:15377"]
    ing4_DI_Water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Tryptone 10.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 ATCC Medium 1065 lists 10.0 g/L Tryptone.
2 Yeast Extract 5.0 G_PER_L FOODON:03315426
yeast extract
 ATCC Medium 1065 lists 5.0 g/L Yeast Extract.
3 Sodium Chloride
Cl.Na 58.443 g/mol
10.0 G_PER_L CHEBI:26710
sodium chloride
 ATCC Medium 1065 lists 10 g/L Sodium Chloride.
4 Agar 15.0 G_PER_L CHEBI:2509
agar
 ATCC Medium 1065 lists 15.0 g/L Agar.
5 DI Water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M2447 lists 1000 ml DI Water for the ATCC LB Agar, Miller formula.

Preparation

  1. Autoclave at 121 degrees C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.513259Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2447
2026-01-28T19:48:38.074692Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:02.028947Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009026
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.787442+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB Agar, Miller" to "lb_agar_miller" for consistent matching
2026-06-05T07:40:36.793803+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m2447_score15.py RESOLVED_TOGO_M2447_SCORE15 TOGO M2447 imports the scratch LB Agar, Miller formulation from ATCC Medium 1065. ATCC lists 10.0 g Tryptone, 5.0 g Yeast Extract, 10 g Sodium Chloride, and 15.0 g Agar; TOGO M2447 retains the one-liter DI Water row. Final pH is 7.0 +/- 0.2. Corrected the imported water unit artifact, grounded all disclosed components, recorded final pH 7.0 +/- 0.2 and the 121 degrees C autoclave instruction.