Overview
- External ontology
- TOGO:M2450 — SP4-Z Medium
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- TOGO M2450 / DSMZ Medium 1076b records the DSMZ Medium 1076b SP4-Z branch using glucose as its energy source. DSMZ Medium 1076b is a 200 ml formula that replaces yeast extract from normal SP4 with fish-sperm DNA, adjusts pH to 7.4, autoclaves at 121 degrees C for 15 minutes, aseptically adds CMRL-1066, autoclaved Yeastolate, heat-inactivated fetal bovine serum, and heat-inactivated swine serum, and then adds either glucose or arginine HCl; the two energy sources should not be combined. This liquid branch uses 130 ml distilled water per 200 ml.
Composition
flowchart LR
CultureMech_009030["sp4_z_medium"]
ing0_distilled_water["distilled water"]
CultureMech_009030 --> ing0_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_distilled_water -.-> CHEBI_15377
ing1_DNA__fish_sperm__SERVA_["DNA (fish sperm; SERVA)"]
CultureMech_009030 --> ing1_DNA__fish_sperm__SERVA_
CHEBI_16991["CHEBI:16991"]
ing1_DNA__fish_sperm__SERVA_ -.-> CHEBI_16991
ing2_Bacto_Peptone__BD_["Bacto-Peptone (BD)"]
CultureMech_009030 --> ing2_Bacto_Peptone__BD_
ing3_Tryptone["Tryptone"]
CultureMech_009030 --> ing3_Tryptone
ing4_PPLO_broth["PPLO broth"]
CultureMech_009030 --> ing4_PPLO_broth
ing5_Fetal_bovine_serum__heat_inactivated_["Fetal bovine serum (heat-inactivated)"]
CultureMech_009030 --> ing5_Fetal_bovine_serum__heat_inactivated_
ing6_Yeastolate__BD__2___autoclaved_["Yeastolate (BD; 2%, autoclaved)"]
CultureMech_009030 --> ing6_Yeastolate__BD__2___autoclaved_
ing7_Swine_serum__heat_inactivated_["Swine serum (heat-inactivated)"]
CultureMech_009030 --> ing7_Swine_serum__heat_inactivated_
ing8_CMRL_1066_medium__10x_concentrated__GIBCO_["CMRL-1066 medium (10x concentrated; GIBCO)"]
CultureMech_009030 --> ing8_CMRL_1066_medium__10x_concentrated__GIBCO_
ing9_Glucose__50__aqueous_solution_["Glucose (50% aqueous solution)"]
CultureMech_009030 --> ing9_Glucose__50__aqueous_solution_
CHEBI_17234["CHEBI:17234"]
ing9_Glucose__50__aqueous_solution_ -.-> CHEBI_17234
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
distilled water
H2O 18.015 g/mol
|
650.0 ML_PER_L |
CHEBI:15377
water
|
DSMZ Medium 1076b lists 130 ml distilled water per 200 ml for this SP4-Z branch. |
| 2 | DNA (fish sperm; SERVA) | 0.2 G_PER_L |
CHEBI:16991
deoxyribonucleic acid
|
DSMZ Medium 1076b lists 0.04 g DNA from fish sperm per 200 ml; normalized to 0.2 g/L. |
| 3 | Bacto-Peptone (BD) | 5.0 G_PER_L |
MICRO:0000178 (source link unavailable)
Peptone
|
DSMZ Medium 1076b lists 1.00 g Bacto-Peptone per 200 ml; normalized to 5.0 g/L. |
| 4 | Tryptone | 10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
DSMZ Medium 1076b lists 2.00 g Tryptone per 200 ml; normalized to 10.0 g/L. |
| 5 | PPLO broth | 3.5 G_PER_L | DSMZ Medium 1076b lists 0.70 g PPLO broth per 200 ml; normalized to 3.5 g/L and retained without an ontology grounding as an opaque complex broth product. | |
| 6 | Fetal bovine serum (heat-inactivated) | 85.0 ML_PER_L | DSMZ Medium 1076b lists 17 ml heat-inactivated fetal bovine serum per 200 ml; normalized to 85.0 ml/L and retained without an ontology grounding. | |
| 7 | Yeastolate (BD; 2%, autoclaved) | 100.0 ML_PER_L | DSMZ Medium 1076b lists 20 ml 2% autoclaved Yeastolate per 200 ml; normalized to 100.0 ml/L and retained without an ontology grounding as a commercial yeastolate solution. | |
| 8 | Swine serum (heat-inactivated) | 85.0 ML_PER_L | DSMZ Medium 1076b lists 17 ml heat-inactivated swine serum per 200 ml; normalized to 85.0 ml/L and retained without an ontology grounding. | |
| 9 | CMRL-1066 medium (10x concentrated; GIBCO) | 50.0 ML_PER_L | DSMZ Medium 1076b lists 10 ml 10x CMRL-1066 per 200 ml; normalized to 50.0 ml/L and retained without an ontology grounding as an opaque commercial basal medium. | |
| 10 |
Glucose (50% aqueous solution)
C6H12O6 180.156 g/mol
|
10.0 ML_PER_L |
CHEBI:17234
glucose
|
DSMZ Medium 1076b lists 2 ml of the selected 50% glucose solution per 200 ml; normalized to 10.0 ml/L. |
Preparation
- Adjust pH to 7.4.
- Autoclave at 121 degrees C for 15 minutes.
- Add CMRL-1066 medium, autoclaved Yeastolate, heat-inactivated fetal bovine serum, heat-inactivated swine serum, and the glucose energy source aseptically.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.515665Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2450 |
| 2026-01-28T19:48:16.058238Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:26.495846+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:02.046338Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009030 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.805258+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SP4-Z Medium" to "sp4_z_medium" for consistent matching |
| 2026-05-16T09:03:13.540165+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.811615+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m2450_m2453_sp4z_score15.py | RESOLVED_TOGO_M2450_M2453_SP4Z_SCORE15 | TOGO M2450 / DSMZ Medium 1076b records the DSMZ Medium 1076b SP4-Z branch using glucose as its energy source. DSMZ Medium 1076b is a 200 ml formula that replaces yeast extract from normal SP4 with fish-sperm DNA, adjusts pH to 7.4, autoclaves at 121 degrees C for 15 minutes, aseptically adds CMRL-1066, autoclaved Yeastolate, heat-inactivated fetal bovine serum, and heat-inactivated swine serum, and then adds either glucose or arginine HCl; the two energy sources should not be combined. This liquid branch uses 130 ml distilled water per 200 ml. Corrected TOGO's 200 ml source amounts that had been imported as per-liter concentrations, moved the energy-source solution into the ingredient list as a 10.0 ml/L addition, grounded disclosed simple components and protein hydrolysates, kept opaque product and serum additions intentionally unmapped, and linked the record to DSMZ Medium 1076b SP4-Z. |