sp4_z_medium

CultureMech:009031 bacterial COMPLEX LIQUID
Original name: SP4-Z Medium

Overview

External ontology
TOGO:M2451 — SP4-Z Medium
Applications
  • Microbial cultivation
pH
7.4
Notes
TOGO M2451 / DSMZ Medium 1076b records the DSMZ Medium 1076b SP4-Z branch using arginine as its energy source. DSMZ Medium 1076b is a 200 ml formula that replaces yeast extract from normal SP4 with fish-sperm DNA, adjusts pH to 7.4, autoclaves at 121 degrees C for 15 minutes, aseptically adds CMRL-1066, autoclaved Yeastolate, heat-inactivated fetal bovine serum, and heat-inactivated swine serum, and then adds either glucose or arginine HCl; the two energy sources should not be combined. This liquid branch uses 130 ml distilled water per 200 ml.

Composition

flowchart LR
    CultureMech_009031["sp4_z_medium"]
    ing0_distilled_water["distilled water"]
    CultureMech_009031 --> ing0_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_distilled_water -.-> CHEBI_15377
    ing1_DNA__fish_sperm__SERVA_["DNA (fish sperm; SERVA)"]
    CultureMech_009031 --> ing1_DNA__fish_sperm__SERVA_
    CHEBI_16991["CHEBI:16991"]
    ing1_DNA__fish_sperm__SERVA_ -.-> CHEBI_16991
    ing2_Bacto_Peptone__BD_["Bacto-Peptone (BD)"]
    CultureMech_009031 --> ing2_Bacto_Peptone__BD_
    ing3_Tryptone["Tryptone"]
    CultureMech_009031 --> ing3_Tryptone
    ing4_PPLO_broth["PPLO broth"]
    CultureMech_009031 --> ing4_PPLO_broth
    ing5_Fetal_bovine_serum__heat_inactivated_["Fetal bovine serum (heat-inactivated)"]
    CultureMech_009031 --> ing5_Fetal_bovine_serum__heat_inactivated_
    ing6_Yeastolate__BD__2___autoclaved_["Yeastolate (BD; 2%, autoclaved)"]
    CultureMech_009031 --> ing6_Yeastolate__BD__2___autoclaved_
    ing7_Swine_serum__heat_inactivated_["Swine serum (heat-inactivated)"]
    CultureMech_009031 --> ing7_Swine_serum__heat_inactivated_
    ing8_CMRL_1066_medium__10x_concentrated__GIBCO_["CMRL-1066 medium (10x concentrated; GIBCO)"]
    CultureMech_009031 --> ing8_CMRL_1066_medium__10x_concentrated__GIBCO_
    ing9_Arginine_HCl__50__aqueous_solution_["Arginine HCl (50% aqueous solution)"]
    CultureMech_009031 --> ing9_Arginine_HCl__50__aqueous_solution_
    CHEBI_31235["CHEBI:31235"]
    ing9_Arginine_HCl__50__aqueous_solution_ -.-> CHEBI_31235

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 distilled water
H2O 18.015 g/mol
650.0 ML_PER_L CHEBI:15377
water
 DSMZ Medium 1076b lists 130 ml distilled water per 200 ml for this SP4-Z branch.
2 DNA (fish sperm; SERVA) 0.2 G_PER_L CHEBI:16991
deoxyribonucleic acid
 DSMZ Medium 1076b lists 0.04 g DNA from fish sperm per 200 ml; normalized to 0.2 g/L.
3 Bacto-Peptone (BD) 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 DSMZ Medium 1076b lists 1.00 g Bacto-Peptone per 200 ml; normalized to 5.0 g/L.
4 Tryptone 10.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 DSMZ Medium 1076b lists 2.00 g Tryptone per 200 ml; normalized to 10.0 g/L.
5 PPLO broth 3.5 G_PER_L DSMZ Medium 1076b lists 0.70 g PPLO broth per 200 ml; normalized to 3.5 g/L and retained without an ontology grounding as an opaque complex broth product.
6 Fetal bovine serum (heat-inactivated) 85.0 ML_PER_L DSMZ Medium 1076b lists 17 ml heat-inactivated fetal bovine serum per 200 ml; normalized to 85.0 ml/L and retained without an ontology grounding.
7 Yeastolate (BD; 2%, autoclaved) 100.0 ML_PER_L DSMZ Medium 1076b lists 20 ml 2% autoclaved Yeastolate per 200 ml; normalized to 100.0 ml/L and retained without an ontology grounding as a commercial yeastolate solution.
8 Swine serum (heat-inactivated) 85.0 ML_PER_L DSMZ Medium 1076b lists 17 ml heat-inactivated swine serum per 200 ml; normalized to 85.0 ml/L and retained without an ontology grounding.
9 CMRL-1066 medium (10x concentrated; GIBCO) 50.0 ML_PER_L DSMZ Medium 1076b lists 10 ml 10x CMRL-1066 per 200 ml; normalized to 50.0 ml/L and retained without an ontology grounding as an opaque commercial basal medium.
10 Arginine HCl (50% aqueous solution)
C6H14N4O2.HCl 210.665 g/mol
10.0 ML_PER_L CHEBI:31235
L-Arginine x HCl
 DSMZ Medium 1076b lists 2 ml of the selected 50% arginine solution per 200 ml; normalized to 10.0 ml/L.

Preparation

  1. Adjust pH to 7.4.
  2. Autoclave at 121 degrees C for 15 minutes.
  3. Add CMRL-1066 medium, autoclaved Yeastolate, heat-inactivated fetal bovine serum, heat-inactivated swine serum, and the arginine energy source aseptically.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.517113Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2451
2026-01-28T19:48:20.490233Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:33.952309+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:02.051900Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009031
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.810491+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SP4-Z Medium" to "sp4_z_medium" for consistent matching
2026-05-16T09:03:13.546721+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:36.817766+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m2450_m2453_sp4z_score15.py RESOLVED_TOGO_M2450_M2453_SP4Z_SCORE15 TOGO M2451 / DSMZ Medium 1076b records the DSMZ Medium 1076b SP4-Z branch using arginine as its energy source. DSMZ Medium 1076b is a 200 ml formula that replaces yeast extract from normal SP4 with fish-sperm DNA, adjusts pH to 7.4, autoclaves at 121 degrees C for 15 minutes, aseptically adds CMRL-1066, autoclaved Yeastolate, heat-inactivated fetal bovine serum, and heat-inactivated swine serum, and then adds either glucose or arginine HCl; the two energy sources should not be combined. This liquid branch uses 130 ml distilled water per 200 ml. Corrected TOGO's 200 ml source amounts that had been imported as per-liter concentrations, moved the energy-source solution into the ingredient list as a 10.0 ml/L addition, grounded disclosed simple components and protein hydrolysates, kept opaque product and serum additions intentionally unmapped, and linked the record to DSMZ Medium 1076b SP4-Z.