sp4_z_medium

CultureMech:009033 bacterial COMPLEX SOLID_AGAR
Original name: SP4-Z Medium

Overview

External ontology
TOGO:M2453 — SP4-Z Medium
Applications
  • Microbial cultivation
pH
7.4
Notes
TOGO M2453 / DSMZ Medium 1076b records the DSMZ Medium 1076b SP4-Z branch using arginine as its energy source. DSMZ Medium 1076b is a 200 ml formula that replaces yeast extract from normal SP4 with fish-sperm DNA, adjusts pH to 7.4, autoclaves at 121 degrees C for 15 minutes, aseptically adds CMRL-1066, autoclaved Yeastolate, heat-inactivated fetal bovine serum, and heat-inactivated swine serum, and then adds either glucose or arginine HCl; the two energy sources should not be combined. This agar-plate branch uses 126 ml distilled water per 200 ml, adds 2 g agar, and is held at 55 degrees C before supplements are added.

Composition

flowchart LR
    CultureMech_009033["sp4_z_medium"]
    ing0_distilled_water["distilled water"]
    CultureMech_009033 --> ing0_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_distilled_water -.-> CHEBI_15377
    ing1_agar["agar"]
    CultureMech_009033 --> ing1_agar
    CHEBI_2509["CHEBI:2509"]
    ing1_agar -.-> CHEBI_2509
    ing2_DNA__fish_sperm__SERVA_["DNA (fish sperm; SERVA)"]
    CultureMech_009033 --> ing2_DNA__fish_sperm__SERVA_
    CHEBI_16991["CHEBI:16991"]
    ing2_DNA__fish_sperm__SERVA_ -.-> CHEBI_16991
    ing3_Bacto_Peptone__BD_["Bacto-Peptone (BD)"]
    CultureMech_009033 --> ing3_Bacto_Peptone__BD_
    ing4_Tryptone["Tryptone"]
    CultureMech_009033 --> ing4_Tryptone
    ing5_PPLO_broth["PPLO broth"]
    CultureMech_009033 --> ing5_PPLO_broth
    ing6_Fetal_bovine_serum__heat_inactivated_["Fetal bovine serum (heat-inactivated)"]
    CultureMech_009033 --> ing6_Fetal_bovine_serum__heat_inactivated_
    ing7_Yeastolate__BD__2___autoclaved_["Yeastolate (BD; 2%, autoclaved)"]
    CultureMech_009033 --> ing7_Yeastolate__BD__2___autoclaved_
    ing8_Swine_serum__heat_inactivated_["Swine serum (heat-inactivated)"]
    CultureMech_009033 --> ing8_Swine_serum__heat_inactivated_
    ing9_CMRL_1066_medium__10x_concentrated__GIBCO_["CMRL-1066 medium (10x concentrated; GIBCO)"]
    CultureMech_009033 --> ing9_CMRL_1066_medium__10x_concentrated__GIBCO_
    ing10_Arginine_HCl__50__aqueous_solution_["Arginine HCl (50% aqueous solution)"]
    CultureMech_009033 --> ing10_Arginine_HCl__50__aqueous_solution_
    CHEBI_31235["CHEBI:31235"]
    ing10_Arginine_HCl__50__aqueous_solution_ -.-> CHEBI_31235

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 distilled water
H2O 18.015 g/mol
630.0 ML_PER_L CHEBI:15377
water
 DSMZ Medium 1076b lists 126 ml distilled water per 200 ml for this SP4-Z branch.
2 agar 10.0 G_PER_L CHEBI:2509
agar
 DSMZ Medium 1076b lists 2 g agar per 200 ml for agar plates; normalized to 10.0 g/L.
3 DNA (fish sperm; SERVA) 0.2 G_PER_L CHEBI:16991
deoxyribonucleic acid
 DSMZ Medium 1076b lists 0.04 g DNA from fish sperm per 200 ml; normalized to 0.2 g/L.
4 Bacto-Peptone (BD) 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
 DSMZ Medium 1076b lists 1.00 g Bacto-Peptone per 200 ml; normalized to 5.0 g/L.
5 Tryptone 10.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 DSMZ Medium 1076b lists 2.00 g Tryptone per 200 ml; normalized to 10.0 g/L.
6 PPLO broth 3.5 G_PER_L DSMZ Medium 1076b lists 0.70 g PPLO broth per 200 ml; normalized to 3.5 g/L and retained without an ontology grounding as an opaque complex broth product.
7 Fetal bovine serum (heat-inactivated) 85.0 ML_PER_L DSMZ Medium 1076b lists 17 ml heat-inactivated fetal bovine serum per 200 ml; normalized to 85.0 ml/L and retained without an ontology grounding.
8 Yeastolate (BD; 2%, autoclaved) 100.0 ML_PER_L DSMZ Medium 1076b lists 20 ml 2% autoclaved Yeastolate per 200 ml; normalized to 100.0 ml/L and retained without an ontology grounding as a commercial yeastolate solution.
9 Swine serum (heat-inactivated) 85.0 ML_PER_L DSMZ Medium 1076b lists 17 ml heat-inactivated swine serum per 200 ml; normalized to 85.0 ml/L and retained without an ontology grounding.
10 CMRL-1066 medium (10x concentrated; GIBCO) 50.0 ML_PER_L DSMZ Medium 1076b lists 10 ml 10x CMRL-1066 per 200 ml; normalized to 50.0 ml/L and retained without an ontology grounding as an opaque commercial basal medium.
11 Arginine HCl (50% aqueous solution)
C6H14N4O2.HCl 210.665 g/mol
10.0 ML_PER_L CHEBI:31235
L-Arginine x HCl
 DSMZ Medium 1076b lists 2 ml of the selected 50% arginine solution per 200 ml; normalized to 10.0 ml/L.

Preparation

  1. Adjust pH to 7.4.
  2. Autoclave at 121 degrees C for 15 minutes.
  3. Hold agar medium at 55 degrees C before adding supplements.
  4. Add CMRL-1066 medium, autoclaved Yeastolate, heat-inactivated fetal bovine serum, heat-inactivated swine serum, and the arginine energy source aseptically.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.520050Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2453
2026-01-28T19:48:11.682994Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:19.179159+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:02.063162Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009033
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.821637+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SP4-Z Medium" to "sp4_z_medium" for consistent matching
2026-05-16T09:03:13.558816+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:36.830773+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m2450_m2453_sp4z_score15.py RESOLVED_TOGO_M2450_M2453_SP4Z_SCORE15 TOGO M2453 / DSMZ Medium 1076b records the DSMZ Medium 1076b SP4-Z branch using arginine as its energy source. DSMZ Medium 1076b is a 200 ml formula that replaces yeast extract from normal SP4 with fish-sperm DNA, adjusts pH to 7.4, autoclaves at 121 degrees C for 15 minutes, aseptically adds CMRL-1066, autoclaved Yeastolate, heat-inactivated fetal bovine serum, and heat-inactivated swine serum, and then adds either glucose or arginine HCl; the two energy sources should not be combined. This agar-plate branch uses 126 ml distilled water per 200 ml, adds 2 g agar, and is held at 55 degrees C before supplements are added. Corrected TOGO's 200 ml source amounts that had been imported as per-liter concentrations, moved the energy-source solution into the ingredient list as a 10.0 ml/L addition, grounded disclosed simple components and protein hydrolysates, kept opaque product and serum additions intentionally unmapped, and linked the record to DSMZ Medium 1076b SP4-Z.