rm_medium

CultureMech:009036 bacterial COMPLEX SOLID_AGAR
Original name: RM Medium

Overview

External ontology
TOGO:M2458 — RM Medium
Applications
  • Microbial cultivation
pH
6.0
Notes
ATCC Medium 1341, mirrored by TOGO M2458, lists RM Medium with 20.0 g glucose, 10.0 g yeast extract, 2.0 g K2HPO4, 15.0 g agar if needed, and 1000.0 ml DI water. ATCC directs separately autoclaving each component, combining the sterile components, and adjusting pH to 6.0.

Composition

flowchart LR
    CultureMech_009036["rm_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_009036 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_009036 --> ing1_Yeast_extract
    ing2_K2HPO4["K2HPO4"]
    CultureMech_009036 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_009036 --> ing3_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_needed_ -.-> CHEBI_2509
    ing4_DI_water["DI water"]
    CultureMech_009036 --> ing4_DI_water
    CHEBI_15377["CHEBI:15377"]
    ing4_DI_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
20.0 G_PER_L CHEBI:17234
glucose
 ATCC Medium 1341 / TOGO M2458 lists 20.0 g/L glucose.
2 Yeast extract 10.0 G_PER_L FOODON:03315426
yeast extract
 ATCC Medium 1341 / TOGO M2458 lists 10.0 g/L yeast extract.
3 K2HPO4
HO4P.2K 174.174 g/mol
2.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 ATCC Medium 1341 / TOGO M2458 lists 2.0 g/L K2HPO4.
4 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 ATCC Medium 1341 / TOGO M2458 lists 15.0 g/L agar if needed.
5 DI water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 ATCC Medium 1341 / TOGO M2458 lists 1000.0 ml DI water.

Preparation

  1. Autoclave glucose, yeast extract, K2HPO4, agar if needed, and DI water as separate components.
  2. Combine the separately autoclaved components.
  3. Adjust pH to 6.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.529517Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2458
2026-01-28T19:48:42.029366Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:02.099321Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009036
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.862065+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RM Medium" to "rm_medium" for consistent matching
2026-06-05T07:41:44.566648+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.047852+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00 repair_togo_m2458_rm_score15.py RESOLVED_TOGO_M2458_RM_SCORE15 Corrected the imported DI-water row from a g/L artifact to 1000.0 ml/L, grounded all five components, added ATCC's pH 6.0 and separate-autoclaving instructions, and marked the formula as curated.