Overview
- External ontology
- TOGO:M2461 — Methylophaga Medium 1403
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2461 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1337.pdf
Composition
flowchart LR
CultureMech_009040["methylophaga_medium_1403"]
ing0_methanol["methanol"]
CultureMech_009040 --> ing0_methanol
CHEBI_17790["CHEBI:17790"]
ing0_methanol -.-> CHEBI_17790
ing1_Distilled_water["Distilled water"]
CultureMech_009040 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009040 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaCl["NaCl"]
CultureMech_009040 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009040 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_009040 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2["CaCl2"]
CultureMech_009040 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_Bis_Tris["Bis-Tris"]
CultureMech_009040 --> ing7_Bis_Tris
CHEBI_41250["CHEBI:41250"]
ing7_Bis_Tris -.-> CHEBI_41250
ing8_KH2PO4["KH2PO4"]
CultureMech_009040 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_NH4Cl["NH4Cl"]
CultureMech_009040 --> ing9_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing9_NH4Cl -.-> CHEBI_31206
ing10_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_009040 --> ing10_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing10_Ferric_ammonium_citrate -.-> CHEBI_31604
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009040 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_AlK_SO4_2["AlK(SO4)2"]
CultureMech_009040 --> ing12_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing12_AlK_SO4_2 -.-> CHEBI_86463
ing13_MgSO4["MgSO4"]
CultureMech_009040 --> ing13_MgSO4
CHEBI_32599["CHEBI:32599"]
ing13_MgSO4 -.-> CHEBI_32599
ing14_CuSO4["CuSO4"]
CultureMech_009040 --> ing14_CuSO4
CHEBI_23414["CHEBI:23414"]
ing14_CuSO4 -.-> CHEBI_23414
ing15_CoCl2["CoCl2"]
CultureMech_009040 --> ing15_CoCl2
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2 -.-> CHEBI_35696
ing16_FeSO4["FeSO4"]
CultureMech_009040 --> ing16_FeSO4
CHEBI_75832["CHEBI:75832"]
ing16_FeSO4 -.-> CHEBI_75832
ing17_Na2MoO4["Na2MoO4"]
CultureMech_009040 --> ing17_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing17_Na2MoO4 -.-> CHEBI_75215
ing18_MnSO4["MnSO4"]
CultureMech_009040 --> ing18_MnSO4
CHEBI_86360["CHEBI:86360"]
ing18_MnSO4 -.-> CHEBI_86360
ing19_ZnSO4["ZnSO4"]
CultureMech_009040 --> ing19_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing19_ZnSO4 -.-> CHEBI_35176
ing20_KOH["KOH"]
CultureMech_009040 --> ing20_KOH
CHEBI_32035["CHEBI:32035"]
ing20_KOH -.-> CHEBI_32035
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
methanol
CH4O 32.042 g/mol
|
3 G_PER_L |
CHEBI:17790
methanol
|
Role: Solvating media; Properties: Organic compound, Defined component, Simple component |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1200.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1000.0, 100.0, 100.0] |
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
24.1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 24.0, 0.1] |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
11.0 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 10.0] |
| 8 |
Bis-Tris
C8H19NO5 209.242 g/mol
|
10.5 G_PER_L |
CHEBI:41250
bis-tris
|
Role: Buffer [Merged 2 duplicates: 0.5, 10.0] |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.7 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NH4Cl
Cl.H4N 53.492 g/mol
|
10 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Ferric ammonium citrate | 0.3 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.15 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 13 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
1 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.3 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuSO4
Cu.O4S 159.609 g/mol
|
1 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2
2Cl.Co 129.839 g/mol
|
10 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeSO4
Fe.O4S 151.908 g/mol
|
10 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
1 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 19 |
MnSO4
Mn.O4S 151.001 g/mol
|
5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4
O4S.Zn 161.453 g/mol
|
10 G_PER_L |
CHEBI:35176
zinc sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
vitamin B12 solution
Dose: 10 G_PER_L
See mediadive_6105_Vitamin_B12_solution.yaml for composition
Solution T
Dose: 20 G_PER_L
See mediadive_2679_Solution_T.yaml for composition
Mineral Salts Solution
Dose: 1 G_PER_L
See mediadive_3221_Mineral_salts_solution.yaml for composition
Wolfe’s Mineral Solution (see below)
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.537125Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2461 |
| 2026-01-28T19:48:21.527619Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:36.005367+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:05.376539Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:02.143865Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009040 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.909370+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methylophaga Medium 1403" to "methylophaga_medium_1403" for consistent matching |
| 2026-05-16T09:04:23.121702+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:33.299492+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:23.855645+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:07.624017+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |