hi_plus_medium_w_ye_3

CultureMech:009044 bacterial COMPLEX SOLID_AGAR
Original name: HI Plus Medium w/ YE #3

Overview

External ontology
TOGO:M2467 — HI Plus Medium w/ YE #3
Applications
  • Microbial cultivation
pH
7.4
Notes
TOGO M2467 cites ATCC Medium 243; ATCC Medium 243 defines HI Plus Medium w/ YE #3 as Heart Infusion Broth base supplemented with heat-inactivated horse serum and Yeast Extract #3.

Composition

flowchart LR
    CultureMech_009044["hi_plus_medium_w_ye_3"]
    ing0_Heart_Infusion_Broth["Heart Infusion Broth"]
    CultureMech_009044 --> ing0_Heart_Infusion_Broth
    ing1_Agar_Noble__if_required_["Agar Noble (if required)"]
    CultureMech_009044 --> ing1_Agar_Noble__if_required_
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar_Noble__if_required_ -.-> CHEBI_2509
    ing2_DI_Water["DI Water"]
    CultureMech_009044 --> ing2_DI_Water
    CHEBI_15377["CHEBI:15377"]
    ing2_DI_Water -.-> CHEBI_15377
    ing3_Horse_serum__heat_inactivated_["Horse serum (heat-inactivated)"]
    CultureMech_009044 --> ing3_Horse_serum__heat_inactivated_

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Heart Infusion Broth 25.0 G_PER_L ATCC Medium 243 lists 25.0 g Heart Infusion Broth in the base medium.
2 Agar Noble (if required) 15.0 G_PER_L CHEBI:2509
agar
 ATCC Medium 243 lists 15.0 g Agar Noble if a solid medium is required.
3 DI Water
H2O 18.015 g/mol
700.0 ML_PER_L CHEBI:15377
water
 ATCC Medium 243 lists 700.0 mL deionized water.
4 Horse serum (heat-inactivated) 200.0 ML_PER_L ATCC Medium 243 adds 200.0 mL heat-inactivated horse serum after the base medium cools to 55 C.

Preparation

  1. Prepare the base from Heart Infusion Broth, optional Agar Noble, and 700 mL DI water.
  2. Autoclave the base medium at 121 C and cool to 55 C.
  3. Heat-inactivate horse serum at 56 C for 30 minutes.
  4. Warm the serum and Yeast Extract #3 to 55 C, then aseptically add them to the basal medium.

Solutions

Yeast Extract #3

Dose: 100.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Yeast 150.0 G_PER_L ATCC Medium 243 prepares Yeast Extract #3 from 150.0 g store-bought Fleischmann's yeast per 1000.0 mL DI water.
DI Water 1000.0 ML_PER_L ATCC Medium 243 Yeast Extract #3 lists 1000.0 mL deionized water.
ATCC Medium 243 adds 100.0 mL Yeast Extract #3 per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.548947Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2467
2026-03-13T03:09:34.190191Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:02.189065Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009044
2026-03-17T19:46:20.956762+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HI Plus Medium w/ YE #3" to "hi_plus_medium_w_ye_3" for consistent matching
2026-09-07T00:00:00-07:00 repair_togo_exact_score25.py RESOLVED_TOGO_EXACT_SCORE25_GRAPH TOGO M2467 cites ATCC Medium 243; ATCC Medium 243 defines HI Plus Medium w/ YE #3 as Heart Infusion Broth base supplemented with heat-inactivated horse serum and Yeast Extract #3.