Overview
- External ontology
- TOGO:M2474 — Medium Modified For Dechloromonas SPP.
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2474 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium944a.pdf
Composition
flowchart LR
CultureMech_009050["medium_modified_for_dechloromonas_spp"]
ing0_Distilled_water["Distilled water"]
CultureMech_009050 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_009050 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Resazurin["Resazurin"]
CultureMech_009050 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Na2HPO4["Na2HPO4"]
CultureMech_009050 --> ing3_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing3_Na2HPO4 -.-> CHEBI_34683
ing4_NaClO3["NaClO3"]
CultureMech_009050 --> ing4_NaClO3
CHEBI_65242["CHEBI:65242"]
ing4_NaClO3 -.-> CHEBI_65242
ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009050 --> ing5_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing5_Carbon_dioxide_gas -.-> CHEBI_16526
ing6_Nitrogen_gas["Nitrogen gas"]
CultureMech_009050 --> ing6_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing6_Nitrogen_gas -.-> CHEBI_17997
ing7_CaCl2["CaCl2"]
CultureMech_009050 --> ing7_CaCl2
CHEBI_3312["CHEBI:3312"]
ing7_CaCl2 -.-> CHEBI_3312
ing8_MgCl2["MgCl2"]
CultureMech_009050 --> ing8_MgCl2
CHEBI_6636["CHEBI:6636"]
ing8_MgCl2 -.-> CHEBI_6636
ing9_NaHCO3["NaHCO3"]
CultureMech_009050 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Na2SO4["Na2SO4"]
CultureMech_009050 --> ing10_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing10_Na2SO4 -.-> CHEBI_32149
ing11_NH4HCO3["NH4HCO3"]
CultureMech_009050 --> ing11_NH4HCO3
CHEBI_184335["CHEBI:184335"]
ing11_NH4HCO3 -.-> CHEBI_184335
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009050 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_009050 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009050 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009050 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009050 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009050 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_009050 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
CultureMech_009050 --> ing19_HCl__25___7_7_M_
ing20_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_009050 --> ing20_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing20_FeCl2_x_4_H2O -.-> CHEBI_86249
ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_009050 --> ing21_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_009050 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing23_NaOH["NaOH"]
CultureMech_009050 --> ing23_NaOH
CHEBI_32145["CHEBI:32145"]
ing23_NaOH -.-> CHEBI_32145
ing24_Biotin["Biotin"]
CultureMech_009050 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_p_aminobenzoic_acid["p-aminobenzoic acid"]
CultureMech_009050 --> ing25_p_aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_aminobenzoic_acid -.-> CHEBI_30753
ing26_Folic_acid["Folic acid"]
CultureMech_009050 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_009050 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_Riboflavin["Riboflavin"]
CultureMech_009050 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_009050 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_009050 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
4010.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 900.0, 50.0, 50.0, 990.0, 1000.0, 1000.0, 20.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.41 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 4 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.53 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaClO3
ClO3.Na 106.44 g/mol
|
1.06 G_PER_L |
CHEBI:65242
sodium chlorate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 7 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 8 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.11 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.1 G_PER_L |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.73 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.2 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NH4HCO3
CHO3.H4N 79.055 g/mol
|
0.44 G_PER_L |
CHEBI:184335
Ammonium bicarbonate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
190 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 | HCl (25%; 7.7 M) | 10 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Solution | |
| 21 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
NaOH
HO.Na 39.997 g/mol
|
500 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
p-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
50 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
20 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 | Vitamin B12 | 50 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
50 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
25 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
25 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 32 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
50 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
50 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Pyridoxamine-HCl
C8H12N2O2.HCl 204.657 g/mol
|
10 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 |
α-lipoic acid
C8H14O2S2 206.332 g/mol
|
50 G_PER_L |
CHEBI:43796
(S)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 36 |
benzene
C6H6 78.114 g/mol
|
31.2 G_PER_L |
CHEBI:16716
benzene
|
Role: Carbon source; Properties: Defined component, Organic compound, Solution |
Solutions
Solution A:
Dose: 900 G_PER_L
Solution B:
Dose: 50 G_PER_L
Solution C:
Dose: 50 G_PER_L
Vitamin solution:
Dose: 1 G_PER_L
benzene solution
Dose: 20 G_PER_L
Trace element solution SL-10
Dose: 1 G_PER_L
See mediadive_4178_Trace_element_solution_SL-10.yaml for composition
Selenite-Tungstate solution
Dose: 4 G_PER_L
See mediadive_5543_Selenite-tungstate_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.557472Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2474 |
| 2026-01-28T19:48:33.380817Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:55.347082+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:09:26.852391Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 10 duplicate ingredient(s) |
| 2026-03-13T06:20:02.240428Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009050 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.010765+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium Modified For Dechloromonas SPP." to "medium_modified_for_dechloromonas_spp" for consistent matching |
| 2026-05-16T09:03:13.703991+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.973731+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.077166+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:22.542168+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |