Overview
- External ontology
- TOGO:M2482 — MN Marine medium (ATCC Medium 957) with 20 mcg/L of vitamin B12
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2482 Original URL: https://www.atcc.org/~/media/1B9FD4672C2B46019C7294BFBCD33998.ashx
Composition
flowchart LR
CultureMech_009056["mn_marine_medium_atcc_medium_957_with_20_mcg_l_of_vitamin_b12"]
ing0_Distilled_water["Distilled water"]
CultureMech_009056 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009056 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_CaCl2___2H2O["CaCl2 · 2H2O"]
CultureMech_009056 --> ing2_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2___2H2O -.-> CHEBI_86158
ing3_Na2CO3["Na2CO3"]
CultureMech_009056 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_NaNO3["NaNO3"]
CultureMech_009056 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_Seawater["Seawater"]
CultureMech_009056 --> ing5_Seawater
ing6_K2HPO4___3_H2O["K2HPO4 · 3 H2O"]
CultureMech_009056 --> ing6_K2HPO4___3_H2O
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4___3_H2O -.-> CHEBI_131527
ing7_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_009056 --> ing7_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing7_Ferric_ammonium_citrate -.-> CHEBI_31604
ing8_Agar_Noble__Difco_0142___if_needed_["Agar Noble (Difco 0142) (if needed)"]
CultureMech_009056 --> ing8_Agar_Noble__Difco_0142___if_needed_
ing9_Citric_acid["Citric acid"]
CultureMech_009056 --> ing9_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing9_Citric_acid -.-> CHEBI_30769
ing10_EDTA["EDTA"]
CultureMech_009056 --> ing10_EDTA
CHEBI_4735["CHEBI:4735"]
ing10_EDTA -.-> CHEBI_4735
ing11_Trace_Metals_A_5__see_below_["Trace Metals A-5 (see below)"]
CultureMech_009056 --> ing11_Trace_Metals_A_5__see_below_
ing12_KOH["KOH"]
CultureMech_009056 --> ing12_KOH
CHEBI_32035["CHEBI:32035"]
ing12_KOH -.-> CHEBI_32035
ing13_Na2MoO4___2H2O["Na2MoO4 · 2H2O"]
CultureMech_009056 --> ing13_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4___2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_009056 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_MnCl2___4H2O["MnCl2 · 4H2O"]
CultureMech_009056 --> ing15_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2___4H2O -.-> CHEBI_86368
ing16_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_009056 --> ing16_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_7H2O -.-> CHEBI_32312
ing17_CuSO4___5H2O["CuSO4 · 5H2O"]
CultureMech_009056 --> ing17_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4___5H2O -.-> CHEBI_31440
ing18_Co_NO3_2___6H2O["Co(NO3)2 · 6H2O"]
CultureMech_009056 --> ing18_Co_NO3_2___6H2O
CHEBI_86214["CHEBI:86214"]
ing18_Co_NO3_2___6H2O -.-> CHEBI_86214
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
251.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 250.0, 1.0] |
| 2 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.04 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2 · 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.02 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
0.75 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 | Seawater | 750 G_PER_L | Role: Solvating media, Mineral source; Properties: Undefined component | |
| 7 |
K2HPO4 · 3 H2O
HO4P.2K 174.174 g/mol
|
0.02 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Ferric ammonium citrate | 3 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 9 | Agar Noble (Difco 0142) (if needed) | 10 G_PER_L | Role: Solidifying component; Properties: Defined component | |
| 10 |
Citric acid
C6H8O7 192.123 g/mol
|
3 G_PER_L |
CHEBI:30769
citric acid
|
Properties: Defined component, Organic compound, Simple component |
| 11 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Simple component, Organic compound |
| 12 | Trace Metals A-5 (see below) | 1 G_PER_L | ||
| 13 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 14 |
Na2MoO4 · 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.039 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnCl2 · 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuSO4 · 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.079 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Co(NO3)2 · 6H2O
Co.6H2O.2NO3 291.031 g/mol
|
49.4 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.574028Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2482 |
| 2026-01-28T19:48:26.985094Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-13T03:09:15.067302Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:02.311428Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009056 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.083513+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MN Marine medium (ATCC Medium 957) with 20 mcg/L of vitamin B12" to "mn_marine_medium_atcc_medium_957_with_20_mcg_l_of_vitamin_b12" for consistent matching |
| 2026-05-16T09:04:24.783551+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:35.849346+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:25.435465+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.401807+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |