Overview
- External ontology
- TOGO:M248 — Columbia Blood Agar With 5% Sheep Blood
- Applications
-
- Microbial cultivation
- Notes
- TOGO M248 mirrors JCM Medium 256: prepare Columbia blood agar base (Oxoid CM331), sterilize and cool it to about 45 C, add sterile defibrinated sheep blood to 5% final, then dispense into sterile tubes or petri dishes.
Composition
flowchart LR
CultureMech_009063["columbia_blood_agar_with_5_sheep_blood"]
ing0_Columbia_blood_agar_base__Oxoid_CM331_["Columbia blood agar base (Oxoid CM331)"]
CultureMech_009063 --> ing0_Columbia_blood_agar_base__Oxoid_CM331_
ing1_Defibrinated_sheep_blood["Defibrinated sheep blood"]
CultureMech_009063 --> ing1_Defibrinated_sheep_blood
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Columbia blood agar base (Oxoid CM331) | 1000 ML_PER_L | TOGO and JCM Medium 256 list one liter of Columbia blood agar base (Oxoid CM331) prepared according to the manufacturer's directions. | |
| 2 | Defibrinated sheep blood | 5 PERCENT_V_V |
UBERON:0000178
blood
|
JCM Medium 256 adds sterile defibrinated sheep blood to 5% final concentration after sterilization and cooling. |
Preparation
- Prepare 1 L Columbia blood agar base (Oxoid CM331) according to the manufacturer's directions.
- Sterilize the base at 121 C for 15 min.
- Cool the sterilized base to about 45 C.
- Aseptically add 5% (final) sterile defibrinated sheep blood.
- Mix and quickly dispense into sterile test tubes or petri dishes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.698671Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M248 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: sterile defibrinated sheep blood |
| 2026-03-13T06:20:02.332825Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009063 |
| 2026-03-17T19:46:21.106015+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Columbia Blood Agar With 5% Sheep Blood" to "columbia_blood_agar_with_5_sheep_blood" for consistent matching |
| 2026-05-16T09:03:13.783665+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-06T00:00:00-07:00 | repair_columbia_blood_score40.py | RESOLVED_COLUMBIA_BLOOD_SCORE40_GRAPH | TOGO M248 mirrors JCM Medium 256: prepare Columbia blood agar base (Oxoid CM331), sterilize and cool it to about 45 C, add sterile defibrinated sheep blood to 5% final, then dispense into sterile tubes or petri dishes. |