Overview
- External ontology
- TOGO:M2491 — MRSsi
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2491
Composition
flowchart LR
CultureMech_009065["mrssi"]
ing0_Sol__1["Sol. 1"]
CultureMech_009065 --> ing0_Sol__1
ing1_xylose["xylose"]
CultureMech_009065 --> ing1_xylose
CHEBI_18222["CHEBI:18222"]
ing1_xylose -.-> CHEBI_18222
ing2_Distilled_water["Distilled water"]
CultureMech_009065 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009065 --> ing3_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4___7H2O -.-> CHEBI_31795
ing4_yeast_extract["yeast extract"]
CultureMech_009065 --> ing4_yeast_extract
ing5_CoCl2___6H2O["CoCl2 · 6H2O"]
CultureMech_009065 --> ing5_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing5_CoCl2___6H2O -.-> CHEBI_35696
ing6_sodium_acetate["sodium acetate"]
CultureMech_009065 --> ing6_sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_sodium_acetate -.-> CHEBI_32954
ing7_Tween_80["Tween 80"]
CultureMech_009065 --> ing7_Tween_80
CHEBI_53426["CHEBI:53426"]
ing7_Tween_80 -.-> CHEBI_53426
ing8_MnSO4___4_H2O["MnSO4 · 4 H2O"]
CultureMech_009065 --> ing8_MnSO4___4_H2O
CHEBI_86358["CHEBI:86358"]
ing8_MnSO4___4_H2O -.-> CHEBI_86358
ing9_casamino_acids["casamino acids"]
CultureMech_009065 --> ing9_casamino_acids
ing10_peptone["peptone"]
CultureMech_009065 --> ing10_peptone
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol. 1 | 5 G_PER_L | ||
| 2 |
xylose
C5H10O5 150.13 g/mol
|
variable VARIABLE |
CHEBI:18222
xylose
|
Role: Carbon source |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 | yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
CoCl2 · 6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
sodium acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 8 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.5 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Simple component, Defined component |
| 9 |
MnSO4 · 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.05 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 10 | casamino acids | 5 G_PER_L | Properties: Undefined component | |
| 11 | peptone | 5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.581643Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2491 |
| 2026-01-28T19:48:34.592561Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: xylose |
| 2026-03-13T06:20:02.340083Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009065 |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.113563+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRSsi" to "mrssi" for consistent matching |
| 2026-05-16T09:04:26.815204+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:38.547576+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.253212+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:27.361972+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |