Overview
- External ontology
- TOGO:M2509 — SOT medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2509 Original URL: https://mcc.nies.go.jp/02medium.html
Composition
flowchart LR
CultureMech_009080["sot_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009080 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009080 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009080 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2___2H2O["CaCl2 · 2H2O"]
CultureMech_009080 --> ing3_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2___2H2O -.-> CHEBI_86158
ing4_K2HPO4["K2HPO4"]
CultureMech_009080 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_FeSO4___7H2O["FeSO4 · 7H2O"]
CultureMech_009080 --> ing5_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4___7H2O -.-> CHEBI_75836
ing6_NaHCO3["NaHCO3"]
CultureMech_009080 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_NaNO3["NaNO3"]
CultureMech_009080 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_K2SO4["K2SO4"]
CultureMech_009080 --> ing8_K2SO4
CHEBI_32036["CHEBI:32036"]
ing8_K2SO4 -.-> CHEBI_32036
ing9_Na2EDTA___2H2O["Na2EDTA · 2H2O"]
CultureMech_009080 --> ing9_Na2EDTA___2H2O
CHEBI_64758["CHEBI:64758"]
ing9_Na2EDTA___2H2O -.-> CHEBI_64758
ing10_CO2["CO2"]
CultureMech_009080 --> ing10_CO2
CHEBI_16526["CHEBI:16526"]
ing10_CO2 -.-> CHEBI_16526
ing11_Na2MoO4___2H2O["Na2MoO4 · 2H2O"]
CultureMech_009080 --> ing11_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4___2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_009080 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_ZnSO4___7H2O["ZnSO4 · 7H2O"]
CultureMech_009080 --> ing13_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4___7H2O -.-> CHEBI_32312
ing14_CuSO4___5H2O["CuSO4 · 5H2O"]
CultureMech_009080 --> ing14_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4___5H2O -.-> CHEBI_31440
ing15_MnSO4___5H2O_1__["MnSO4 · 5H2O 1 )"]
CultureMech_009080 --> ing15_MnSO4___5H2O_1__
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
199.9 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 99.9, 100.0] |
| 2 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2 · 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
50 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
FeSO4 · 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.68 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
250 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
K2SO4
2K.O4S 174.259 g/mol
|
100 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2EDTA · 2H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
8 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 11 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 12 |
Na2MoO4 · 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
2.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
286 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnSO4 · 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
22.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuSO4 · 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
7.9 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 | MnSO4 · 5H2O 1 ) | 217 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
A5 solution
Dose: 0.1 G_PER_L
See mediadive_4987_A5_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.594617Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2509 |
| 2026-01-28T19:48:42.150646Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 |
| 2026-03-09T08:05:11.047983+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:42.873952Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:02.399833Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009080 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.175254+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SOT medium" to "sot_medium" for consistent matching |
| 2026-05-16T09:04:31.885188+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:46.077054+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:32.433213+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:11.558341+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |