Overview
- External ontology
- TOGO:M2520 — R2A Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2520 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf
Composition
flowchart LR
CultureMech_009090["r2a_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009090 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009090 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009090 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_009090 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Na_pyruvate["Na-pyruvate"]
CultureMech_009090 --> ing4_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Na_pyruvate -.-> CHEBI_50144
ing5_Glucose["Glucose"]
CultureMech_009090 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Soluble_starch["Soluble starch"]
CultureMech_009090 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Agar["Agar"]
CultureMech_009090 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Casamino_acids["Casamino acids"]
CultureMech_009090 --> ing8_Casamino_acids
ing9_Proteose_Peptone__Difco_no__3_["Proteose Peptone (Difco no. 3)"]
CultureMech_009090 --> ing9_Proteose_Peptone__Difco_no__3_
ing10_Biotin["Biotin"]
CultureMech_009090 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_p_aminobenzoic_acid["p-aminobenzoic acid"]
CultureMech_009090 --> ing11_p_aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing11_p_aminobenzoic_acid -.-> CHEBI_30753
ing12_Folic_acid["Folic acid"]
CultureMech_009090 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Vitamin_B12["Vitamin B12"]
CultureMech_009090 --> ing13_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing13_Vitamin_B12 -.-> CHEBI_176843
ing14_Riboflavin["Riboflavin"]
CultureMech_009090 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_009090 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_Nicotine_amide["Nicotine amide"]
CultureMech_009090 --> ing16_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing16_Nicotine_amide -.-> CHEBI_17154
ing17_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_009090 --> ing17_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing17_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing18_Pantothenic_acid["Pantothenic acid"]
CultureMech_009090 --> ing18_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing18_Pantothenic_acid -.-> CHEBI_7916
ing19_Pyridoxamine_HCl["Pyridoxamine-HCl"]
CultureMech_009090 --> ing19_Pyridoxamine_HCl
CHEBI_131531["CHEBI:131531"]
ing19_Pyridoxamine_HCl -.-> CHEBI_131531
ing20___lipoic_acid["α-lipoic acid"]
CultureMech_009090 --> ing20___lipoic_acid
CHEBI_43796["CHEBI:43796"]
ing20___lipoic_acid -.-> CHEBI_43796
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1000.0, 1000.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 7 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 | Casamino acids | 0.5 G_PER_L | Properties: Undefined component | |
| 10 | Proteose Peptone (Difco no. 3) | 0.5 G_PER_L | Properties: Undefined component | |
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
p-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
50 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
20 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 | Vitamin B12 | 50 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
50 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
25 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
25 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 18 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
50 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
50 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Pyridoxamine-HCl
C8H12N2O2.HCl 204.657 g/mol
|
10 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
α-lipoic acid
C8H14O2S2 206.332 g/mol
|
50 G_PER_L |
CHEBI:43796
(S)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Solutions
Vitamin solution:
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.601262Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2520 |
| 2026-01-28T19:48:29.864294Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:49.658486+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:20.819672Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:02.436858Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009090 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.210965+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A Medium" to "r2a_medium" for consistent matching |
| 2026-05-16T09:03:13.837365+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:37.087660+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.461982+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:26.885254+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.116835+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:46.739627+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |