Overview
- External ontology
- TOGO:M2521 — Astidious Anaerobe Agar
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.4}
- Notes
- TOGO M2521 lists the DSMZ Medium 1203 Fastidious Anaerobe Agar formulation: 45.7 g Acumedia Fastidious Anaerobe Agar brought to one liter with deionized water, soaked, autoclaved at 121 C, cooled to 47 C, and supplemented with 5-10% sterile defibrinated horse blood.
Composition
flowchart LR
CultureMech_009091["astidious_anaerobe_agar"]
ing0_DI_water["DI water"]
CultureMech_009091 --> ing0_DI_water
CHEBI_15377["CHEBI:15377"]
ing0_DI_water -.-> CHEBI_15377
ing1_Fastidious_Anaerobe_Agar__Acumedia_["Fastidious Anaerobe Agar (Acumedia)"]
CultureMech_009091 --> ing1_Fastidious_Anaerobe_Agar__Acumedia_
ing2_Sterile_defibrinated_horse_blood["Sterile defibrinated horse blood"]
CultureMech_009091 --> ing2_Sterile_defibrinated_horse_blood
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
DI water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M2521 lists 1000 ml deionized water. |
| 2 | Fastidious Anaerobe Agar (Acumedia) | 45.7 G_PER_L | TOGO M2521 lists 45.7 g Fastidious Anaerobe Agar (Acumedia) per liter. | |
| 3 | Sterile defibrinated horse blood | 5-10 PERCENT_V_V |
UBERON:0000178
blood
|
TOGO M2521 instructs adding 5-10% sterile defibrinated horse blood after autoclaving and cooling to 47 C. |
Preparation
- Allow the agar powder to soak for 10 min, then swirl to mix.
- Autoclave the mixed medium at 121 C for 15 min.
- Cool the sterilized medium to 47 C.
- Aseptically add 5-10% sterile defibrinated horse blood, mix well, and pour plates.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.603958Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2521 |
| 2026-03-13T06:20:02.443144Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009091 |
| 2026-03-17T19:46:21.217020+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Astidious Anaerobe Agar" to "astidious_anaerobe_agar" for consistent matching |
| 2026-09-07T00:00:00-07:00 | repair_togo_literal_products_score40.py | RESOLVED_TOGO_LITERAL_PRODUCT_SCORE40_GRAPH | TOGO M2521 lists the DSMZ Medium 1203 Fastidious Anaerobe Agar formulation: 45.7 g Acumedia Fastidious Anaerobe Agar brought to one liter with deionized water, soaked, autoclaved at 121 C, cooled to 47 C, and supplemented with 5-10% sterile defibrinated horse blood. |