Overview
- External ontology
- TOGO:M2522 — Chopped Meat Medium (add Vitamin K1)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2522 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78.pdf
Composition
flowchart LR
CultureMech_009092["chopped_meat_medium_add_vitamin_k1"]
ing0_NaOH_1_N["NaOH 1 N"]
CultureMech_009092 --> ing0_NaOH_1_N
ing1_Distilled_water["Distilled water"]
CultureMech_009092 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Ground_beef__fat_free_["Ground beef (fat free)"]
CultureMech_009092 --> ing2_Ground_beef__fat_free_
ing3_Yeast_extract["Yeast extract"]
CultureMech_009092 --> ing3_Yeast_extract
ing4_K2HPO4["K2HPO4"]
CultureMech_009092 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Resazurin["Resazurin"]
CultureMech_009092 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Casitone["Casitone"]
CultureMech_009092 --> ing6_Casitone
ing7_L_cysteine_hydrochloride["L-cysteine hydrochloride"]
CultureMech_009092 --> ing7_L_cysteine_hydrochloride
ing8_N2["N2"]
CultureMech_009092 --> ing8_N2
CHEBI_17997["CHEBI:17997"]
ing8_N2 -.-> CHEBI_17997
ing9_NaOH["NaOH"]
CultureMech_009092 --> ing9_NaOH
CHEBI_32145["CHEBI:32145"]
ing9_NaOH -.-> CHEBI_32145
ing10_haemin["haemin"]
CultureMech_009092 --> ing10_haemin
CHEBI_50385["CHEBI:50385"]
ing10_haemin -.-> CHEBI_50385
ing11_95__ethanol["95% ethanol"]
CultureMech_009092 --> ing11_95__ethanol
ing12_vitamin_K1["vitamin K1"]
CultureMech_009092 --> ing12_vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing12_vitamin_K1 -.-> CHEBI_18067
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | NaOH 1 N | 25 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1099.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 1000.0, 99.0] |
| 3 | Ground beef (fat free) | 500 G_PER_L | Properties: Undefined component | |
| 4 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component |
| 7 | Casitone | 30 G_PER_L | Properties: Undefined component | |
| 8 | L-cysteine hydrochloride | 0.5 G_PER_L | ||
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 10 |
NaOH
HO.Na 39.997 g/mol
|
1 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Inorganic compound, Solution, Defined component |
| 11 |
haemin
C34H32ClFeN4O4 651.952 g/mol
|
50 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Simple component, Organic compound, Defined component |
| 12 | 95% ethanol | 20 G_PER_L | Role: Solvating media; Properties: Defined component, Organic compound, Simple component | |
| 13 |
vitamin K1
C31H46O2 450.707 g/mol
|
0.1 G_PER_L |
CHEBI:18067
phylloquinone
|
Role: Vitamin; Properties: Organic compound, Solution, Defined component |
Solutions
Haemin solution
Dose: 10 G_PER_L
See mediadive_1628_Haemin_solution.yaml for composition
Vitamin K1 solution
Dose: 10 G_PER_L
See mediadive_2227_Vitamin_K1_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.604691Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2522 |
| 2026-01-28T19:48:40.004642Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:07.351588+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:39.067078Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:02.450009Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009092 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.223306+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chopped Meat Medium (add Vitamin K1)" to "chopped_meat_medium_add_vitamin_k1" for consistent matching |
| 2026-05-16T09:03:27.380317+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:53.430537+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.514915+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |