chopped_meat_medium_add_vitamin_k3_and_agar

CultureMech:009095 bacterial COMPLEX SOLID_AGAR
Original name: Chopped Meat Medium (add Vitamin K3 and agar)

Overview

External ontology
TOGO:M2525 — Chopped Meat Medium (add Vitamin K3 and agar)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2525 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78.pdf

Composition

flowchart LR
    CultureMech_009095["chopped_meat_medium_add_vitamin_k3_and_agar"]
    ing0_NaOH_1_N["NaOH 1 N"]
    CultureMech_009095 --> ing0_NaOH_1_N
    ing1_Distilled_water["Distilled water"]
    CultureMech_009095 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Ground_beef__fat_free_["Ground beef (fat free)"]
    CultureMech_009095 --> ing2_Ground_beef__fat_free_
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_009095 --> ing3_Yeast_extract
    ing4_K2HPO4["K2HPO4"]
    CultureMech_009095 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Resazurin["Resazurin"]
    CultureMech_009095 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_Agar["Agar"]
    CultureMech_009095 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Casitone["Casitone"]
    CultureMech_009095 --> ing7_Casitone
    ing8_L_cysteine_hydrochloride["L-cysteine hydrochloride"]
    CultureMech_009095 --> ing8_L_cysteine_hydrochloride
    ing9_N2["N2"]
    CultureMech_009095 --> ing9_N2
    CHEBI_17997["CHEBI:17997"]
    ing9_N2 -.-> CHEBI_17997
    ing10_NaOH["NaOH"]
    CultureMech_009095 --> ing10_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing10_NaOH -.-> CHEBI_32145
    ing11_haemin["haemin"]
    CultureMech_009095 --> ing11_haemin
    CHEBI_50385["CHEBI:50385"]
    ing11_haemin -.-> CHEBI_50385
    ing12_vitamin_K3["vitamin K3"]
    CultureMech_009095 --> ing12_vitamin_K3
    CHEBI_28869["CHEBI:28869"]
    ing12_vitamin_K3 -.-> CHEBI_28869
    ing13_95__ethanol["95% ethanol"]
    CultureMech_009095 --> ing13_95__ethanol

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 NaOH 1 N 25 G_PER_L Properties: Inorganic compound, Solution, Defined component
2 Distilled water
H2O 18.015 g/mol
1099.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1000.0, 99.0]
3 Ground beef (fat free) 500 G_PER_L Properties: Undefined component
4 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
5 K2HPO4
HO4P.2K 174.174 g/mol
5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
7 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
8 Casitone 30 G_PER_L Properties: Undefined component
9 L-cysteine hydrochloride 0.5 G_PER_L
10 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
11 NaOH
HO.Na 39.997 g/mol
1 G_PER_L CHEBI:32145
sodium hydroxide
 Properties: Defined component, Inorganic compound, Solution
12 haemin
C34H32ClFeN4O4 651.952 g/mol
50 G_PER_L CHEBI:50385
hemin
 Role: Growth factor; Properties: Simple component, Organic compound, Defined component
13 vitamin K3
C11H8O2 172.183 g/mol
5 G_PER_L CHEBI:28869
menadione
 Role: Vitamin; Properties: Organic compound, Solution, Defined component
14 95% ethanol variable VARIABLE Role: Solvating media; Properties: Defined component, Organic compound, Simple component

Solutions

Haemin solution

Dose: 10 G_PER_L

See mediadive_1628_Haemin_solution.yaml for composition

Vitamin K3 solution

Dose: 10 G_PER_L

See mediadive_5540_Vitamin_K3_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.610298Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2525
2026-01-28T19:48:15.670759Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2; Added default concentration for ingredient: 95% ethanol
2026-03-09T08:04:25.843301+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:08:54.362360Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:02.477629Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009095
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:21.250200+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Chopped Meat Medium (add Vitamin K3 and agar)" to "chopped_meat_medium_add_vitamin_k3_and_agar" for consistent matching
2026-05-16T09:03:27.410858+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:53.463759+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.526961+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.