Overview
- External ontology
- TOGO:M252 — EG Medium With 10% NaCl
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M252 Original source: JCM - JCM_M260 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=260
Composition
flowchart LR
CultureMech_009099["eg_medium_with_10_nacl"]
ing0_HCl["HCl"]
CultureMech_009099 --> ing0_HCl
CHEBI_17883["CHEBI:17883"]
ing0_HCl -.-> CHEBI_17883
ing1_Distilled_water["Distilled water"]
CultureMech_009099 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_NaCl["NaCl"]
CultureMech_009099 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Na2HPO4["Na2HPO4"]
CultureMech_009099 --> ing3_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing3_Na2HPO4 -.-> CHEBI_34683
ing4_L__Cystine["L--Cystine"]
CultureMech_009099 --> ing4_L__Cystine
CHEBI_16283["CHEBI:16283"]
ing4_L__Cystine -.-> CHEBI_16283
ing5_Glucose["Glucose"]
CultureMech_009099 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Soluble_starch["Soluble starch"]
CultureMech_009099 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Agar["Agar"]
CultureMech_009099 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009099 --> ing8_Yeast_extract__BD_Difco_
ing9_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_009099 --> ing9_Proteose_peptone_No__3__BD_Difco_
ing10_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009099 --> ing10_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing11_Lab__Lemco_powder__Oxoid_["Lab--Lemco powder (Oxoid)"]
CultureMech_009099 --> ing11_Lab__Lemco_powder__Oxoid_
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
HCl
HCl 36.461 g/mol
|
50 G_PER_L |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
| 2 |
Distilled water
H2O 18.015 g/mol
|
950 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
4 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
L--Cystine
C6H12N2O4S2 240.306 g/mol
|
0.2 G_PER_L |
CHEBI:16283
L-cystine
|
Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
1.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 | Yeast extract (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 10 | Proteose peptone No. 3 (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 11 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 12 | Lab--Lemco powder (Oxoid) | 2.4 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.703025Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M252 |
| 2026-01-28T19:48:25.505756Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:02.557859Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009099 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.326551+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EG Medium With 10% NaCl" to "eg_medium_with_10_nacl" for consistent matching |
| 2026-06-05T07:40:37.146321+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-20T19:01:22.690511+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |