Overview
- External ontology
- TOGO:M2535 — Chopped Meat Medium With Carbohydrates
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2535 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110.pdf
Composition
flowchart LR
CultureMech_009105["chopped_meat_medium_with_carbohydrates"]
ing0_NaOH_1_N["NaOH 1 N"]
CultureMech_009105 --> ing0_NaOH_1_N
ing1_Distilled_water["Distilled water"]
CultureMech_009105 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Ground_beef__fat_free_["Ground beef (fat free)"]
CultureMech_009105 --> ing2_Ground_beef__fat_free_
ing3_Yeast_extract["Yeast extract"]
CultureMech_009105 --> ing3_Yeast_extract
ing4_K2HPO4["K2HPO4"]
CultureMech_009105 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_D_Glucose["D-Glucose"]
CultureMech_009105 --> ing5_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing5_D_Glucose -.-> CHEBI_17634
ing6_Cellobiose["Cellobiose"]
CultureMech_009105 --> ing6_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing6_Cellobiose -.-> CHEBI_17057
ing7_Maltose["Maltose"]
CultureMech_009105 --> ing7_Maltose
CHEBI_18167["CHEBI:18167"]
ing7_Maltose -.-> CHEBI_18167
ing8_Starch__soluble_["Starch (soluble)"]
CultureMech_009105 --> ing8_Starch__soluble_
ing9_Casitone["Casitone"]
CultureMech_009105 --> ing9_Casitone
ing10_L_cysteine_hydrochloride["L-cysteine hydrochloride"]
CultureMech_009105 --> ing10_L_cysteine_hydrochloride
ing11_N2["N2"]
CultureMech_009105 --> ing11_N2
CHEBI_17997["CHEBI:17997"]
ing11_N2 -.-> CHEBI_17997
ing12_NaOH["NaOH"]
CultureMech_009105 --> ing12_NaOH
CHEBI_32145["CHEBI:32145"]
ing12_NaOH -.-> CHEBI_32145
ing13_haemin["haemin"]
CultureMech_009105 --> ing13_haemin
CHEBI_50385["CHEBI:50385"]
ing13_haemin -.-> CHEBI_50385
ing14_95__ethanol["95% ethanol"]
CultureMech_009105 --> ing14_95__ethanol
ing15_vitamin_K3["vitamin K3"]
CultureMech_009105 --> ing15_vitamin_K3
CHEBI_28869["CHEBI:28869"]
ing15_vitamin_K3 -.-> CHEBI_28869
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | NaOH 1 N | 25 G_PER_L | Properties: Inorganic compound, Solution, Defined component | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1198.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 3 duplicates: 1000.0, 99.0, 99.0] |
| 3 | Ground beef (fat free) | 500 G_PER_L | Properties: Undefined component | |
| 4 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
D-Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 7 |
Cellobiose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 8 |
Maltose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 9 | Starch (soluble) | 1 G_PER_L | Role: Carbon source; Properties: Undefined component | |
| 10 | Casitone | 30 G_PER_L | Properties: Undefined component | |
| 11 | L-cysteine hydrochloride | 0.5 G_PER_L | ||
| 12 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 13 |
NaOH
HO.Na 39.997 g/mol
|
1 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 14 |
haemin
C34H32ClFeN4O4 651.952 g/mol
|
50 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Organic compound, Simple component, Defined component |
| 15 | 95% ethanol | 1 G_PER_L | Role: Solvating media; Properties: Defined component, Organic compound, Simple component | |
| 16 |
vitamin K3
C11H8O2 172.183 g/mol
|
5 G_PER_L |
CHEBI:28869
menadione
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
Solutions
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Haemin solution
Dose: 10 G_PER_L
See mediadive_1628_Haemin_solution.yaml for composition
Vitamin K3 solution
Dose: 10 G_PER_L
See mediadive_5540_Vitamin_K3_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.652656Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2535 |
| 2026-01-28T19:48:18.337914Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:30.385441+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:59.355802Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:02.647957Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009105 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.414205+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chopped Meat Medium With Carbohydrates" to "chopped_meat_medium_with_carbohydrates" for consistent matching |
| 2026-05-16T09:03:13.970610+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.221332+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.166673+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |