Overview
- External ontology
- TOGO:M2555 — Chromatium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2555 Original URL: https://www.atcc.org/~/media/1BB5D6DF89424B6691801E5C489B8C30.ashx
Composition
flowchart LR
CultureMech_009124["chromatium_medium"]
ing0_CaCl2["CaCl2"]
CultureMech_009124 --> ing0_CaCl2
CHEBI_3312["CHEBI:3312"]
ing0_CaCl2 -.-> CHEBI_3312
ing1_DI_Water["DI Water"]
CultureMech_009124 --> ing1_DI_Water
ing2_KH2PO4["KH2PO4"]
CultureMech_009124 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_009124 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_MgCl2_x_6H2O["MgCl2 x 6H2O"]
CultureMech_009124 --> ing4_MgCl2_x_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_009124 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Sodium_Ascorbate["Sodium Ascorbate"]
CultureMech_009124 --> ing6_Sodium_Ascorbate
CHEBI_113451["CHEBI:113451"]
ing6_Sodium_Ascorbate -.-> CHEBI_113451
ing7_NaHCO3["NaHCO3"]
CultureMech_009124 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Na2S_x_9H2O["Na2S x 9H2O"]
CultureMech_009124 --> ing8_Na2S_x_9H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9H2O -.-> CHEBI_76209
ing9_N2["N2"]
CultureMech_009124 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
ing10_FeSO4_x_7H2O["FeSO4 x 7H2O"]
CultureMech_009124 --> ing10_FeSO4_x_7H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7H2O -.-> CHEBI_75836
ing11_MnCl2_x_4H2O["MnCl2 x 4H2O"]
CultureMech_009124 --> ing11_MnCl2_x_4H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4H2O -.-> CHEBI_86368
ing12_ZnSO4_x_7H2O["ZnSO4 x 7H2O"]
CultureMech_009124 --> ing12_ZnSO4_x_7H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7H2O -.-> CHEBI_32312
ing13_Ethlyene_Diamine_Tetraacetate["Ethlyene Diamine Tetraacetate"]
CultureMech_009124 --> ing13_Ethlyene_Diamine_Tetraacetate
ing14_Vitamin_B12["Vitamin B12"]
CultureMech_009124 --> ing14_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing14_Vitamin_B12 -.-> CHEBI_176843
ing15_Biotin["Biotin"]
CultureMech_009124 --> ing15_Biotin
CHEBI_15956["CHEBI:15956"]
ing15_Biotin -.-> CHEBI_15956
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009124 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_Nicotinic_Acid["Nicotinic Acid"]
CultureMech_009124 --> ing17_Nicotinic_Acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_Acid -.-> CHEBI_15940
ing18_Thiamin["Thiamin"]
CultureMech_009124 --> ing18_Thiamin
CHEBI_18385["CHEBI:18385"]
ing18_Thiamin -.-> CHEBI_18385
ing19_Pantothenic_acid["Pantothenic acid"]
CultureMech_009124 --> ing19_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing19_Pantothenic_acid -.-> CHEBI_7916
ing20_Pyrodoxamine_HCl["Pyrodoxamine HCl"]
CultureMech_009124 --> ing20_Pyrodoxamine_HCl
ing21_H3BO3["H3BO3"]
CultureMech_009124 --> ing21_H3BO3
CHEBI_33118["CHEBI:33118"]
ing21_H3BO3 -.-> CHEBI_33118
ing22_ZnCl2["ZnCl2"]
CultureMech_009124 --> ing22_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing22_ZnCl2 -.-> CHEBI_49976
ing23_KI["KI"]
CultureMech_009124 --> ing23_KI
CHEBI_8346["CHEBI:8346"]
ing23_KI -.-> CHEBI_8346
ing24_Na_selenite["Na selenite"]
CultureMech_009124 --> ing24_Na_selenite
ing25_KBr["KBr"]
CultureMech_009124 --> ing25_KBr
CHEBI_32030["CHEBI:32030"]
ing25_KBr -.-> CHEBI_32030
ing26_AlCl3["AlCl3"]
CultureMech_009124 --> ing26_AlCl3
CHEBI_30114["CHEBI:30114"]
ing26_AlCl3 -.-> CHEBI_30114
ing27_CoCl2["CoCl2"]
CultureMech_009124 --> ing27_CoCl2
CHEBI_35696["CHEBI:35696"]
ing27_CoCl2 -.-> CHEBI_35696
ing28_SnCl2_x_2H2O["SnCl2 x 2H2O"]
CultureMech_009124 --> ing28_SnCl2_x_2H2O
CHEBI_78074["CHEBI:78074"]
ing28_SnCl2_x_2H2O -.-> CHEBI_78074
ing29_LiCl["LiCl"]
CultureMech_009124 --> ing29_LiCl
CHEBI_48607["CHEBI:48607"]
ing29_LiCl -.-> CHEBI_48607
CultureMech_009124 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.8 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 2 | DI Water | 6932.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 8 duplicates: 1000.0, 32.0, 900.0, 200.0, 1000.0, 100.0, 100.0, 3600.0] | |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.8 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MgCl2 x 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.8 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Sodium Ascorbate
C6H7O6.Na 198.106 g/mol
|
2.4 G_PER_L |
CHEBI:113451
sodium ascorbate
|
Properties: Defined component, Organic compound, Simple component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2S x 9H2O
9H2O.2Na.S 240.182 g/mol
|
3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 11 |
FeSO4 x 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MnCl2 x 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
7.02 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.02, 7.0] |
| 13 |
ZnSO4 x 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | Ethlyene Diamine Tetraacetate | 1.5 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 15 | Vitamin B12 | 2 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.1 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Nicotinic Acid
C6H5NO2 123.111 g/mol
|
2 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Thiamin
C12H17N4OS 265.362 g/mol
|
1 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.5 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 | Pyrodoxamine HCl | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 22 |
H3BO3
H3BO3 61.833 g/mol
|
11 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
KI
I.K 166.002 g/mol
|
1 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 | Na selenite | 0.5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 26 |
KBr
Br.K 119.002 g/mol
|
0.5 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
AlCl3
AlCl3 133.341 g/mol
|
1 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
CoCl2
2Cl.Co 129.839 g/mol
|
1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
SnCl2 x 2H2O
2Cl.2H2O.Sn 225.647 g/mol
|
0.5 G_PER_L |
CHEBI:78074
tin(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
LiCl
Cl.Li 42.394 g/mol
|
0.5 G_PER_L |
CHEBI:48607
lithium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
BaCl2
Ba.2Cl 208.234 g/mol
|
0.5 G_PER_L |
CHEBI:63317
barium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
CuCl2
Cl2Cu 134.452 g/mol
|
1 G_PER_L |
CHEBI:49553
copper(II) chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
NaVO3 x H2O
H2O.Na.O3V 139.946 g/mol
|
0.1 G_PER_L |
CHEBI:132095
sodium metavanadate hydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
NiCl2
Cl2Ni 129.599 g/mol
|
1 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 35 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
0.5 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution 1+2+3
Dose: 120 G_PER_L
Solution 4
Dose: 6 G_PER_L
See mediadive_5181_Solution_4.yaml for composition
Solution 1
Dose: 80 G_PER_L
See mediadive_6108_Solution_1.yaml for composition
Solution 2+3
Dose: 46 G_PER_L
Solution 2
Dose: 100 G_PER_L
See mediadive_6109_Solution_2.yaml for composition
Solution 3
Dose: 900 G_PER_L
See mediadive_6110_Solution_3.yaml for composition
Heavy Metal Solution
Dose: 50 G_PER_L
Vitamin B12 Solution
Dose: 3 G_PER_L
See mediadive_6105_Vitamin_B12_solution.yaml for composition
Vitamin Solution
Dose: 15 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Modified Hogland trace elements solution
Dose: 6 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.711844Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2555 |
| 2026-01-28T19:48:28.258985Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:46.824844+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 10 solution(s) from ingredients |
| 2026-03-13T03:09:17.452514Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 8 duplicate ingredient(s) |
| 2026-03-13T06:20:02.955488Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009124 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.698994+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chromatium Medium" to "chromatium_medium" for consistent matching |
| 2026-05-16T09:03:27.484626+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:53.564452+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.563088+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |