methanocella_conradii_medium

Overview

External ontology

TOGO:M2561 — Methanocella Conradii Medium

Applications

• Microbial cultivation

pH

7.0

Notes

Source: https://togomedium.org/medium/M2561 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1318.pdf Composition verified against https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1318.pdf on 2026-08-25 (SHA-256 f2ed1e926a6f1e63b27c3a3690b0d42a871220ee01e43d3ef5a85643fd1d3834). Direct quantities and stock additions were scaled from the printed 1004 ml batch to a one-litre final basis.

Composition

flowchart LR
    CultureMech_009130["methanocella_conradii_medium"]
    ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_009130 --> ing0_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_009130 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_NH4Cl["NH4Cl"]
    CultureMech_009130 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009130 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_009130 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_009130 --> ing5_Yeast_extract
    ing6_Na_acetate["Na-acetate"]
    CultureMech_009130 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7_Na2CO3["Na2CO3"]
    CultureMech_009130 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_009130 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_DL_Dithiothreitol["DL-Dithiothreitol"]
    CultureMech_009130 --> ing9_DL_Dithiothreitol
    CHEBI_18320["CHEBI:18320"]
    ing9_DL_Dithiothreitol -.-> CHEBI_18320
    ing10_Distilled_water["Distilled water"]
    CultureMech_009130 --> ing10_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing10_Distilled_water -.-> CHEBI_15377

Ingredients (11)

Preparation

1. Dissolve all ingredients except carbonate, vitamins, cysteine, and DTT. Include SL-10, selenite-tungstate, and resazurin stocks.
2. Sparge with 80% N2 and 20% CO2 for 30-45 minutes, dispense under that atmosphere to 30% of vessel volume, and autoclave.
3. Add filter-sterilized vitamin stocks and sterile anoxic cysteine and DTT stocks prepared under 100% N2. Add carbonate from a sterile anoxic stock prepared under 80% N2 and 20% CO2.
4. The complete medium should have pH 7.0.
5. After inoculation, pressurize vials to 1 bar overpressure with sterile 100% H2 gas.
6. For SL-10, first dissolve FeCl2 in 25% HCl, dilute in water, add the other salts, and make to 1 litre.

Solutions

Target organisms

No target organisms are recorded.

Provenance