etsa_medium (CultureMech:009136)

Overview

External ontology

TOGO:M2568 — ETSA medium

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2568 Original URL: https://www.atcc.org/~/media/AE1BC225B681490BA78D21448457E756.ashx Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_009136["etsa_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009136 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_009136 --> ing1_Yeast_extract
    ing2_KNO3["KNO3"]
    CultureMech_009136 --> ing2_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing2_KNO3 -.-> CHEBI_63043
    ing3_Sodium_succinate["Sodium succinate"]
    CultureMech_009136 --> ing3_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing3_Sodium_succinate -.-> CHEBI_63675
    ing4_Sodium_lactate__60__syrup["Sodium lactate, 60% syrup"]
    CultureMech_009136 --> ing4_Sodium_lactate__60__syrup
    ing5_Agar["Agar"]
    CultureMech_009136 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Sodium_formate["Sodium formate"]
    CultureMech_009136 --> ing6_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing6_Sodium_formate -.-> CHEBI_62965
    ing7_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_009136 --> ing7_Pancreatic_digest_of_casein
    ing8_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_009136 --> ing8_Peptic_digest_of_soybean_meal
    ing9_Glucose["Glucose"]
    CultureMech_009136 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_Sodium_chloride["Sodium chloride"]
    CultureMech_009136 --> ing10_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing10_Sodium_chloride -.-> CHEBI_26710
    ing11_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_009136 --> ing11_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing11_Dipotassium_phosphate -.-> CHEBI_131527
    ing12_Agar["Agar"]
    CultureMech_009136 --> ing12_Agar
    ing12_Agar -.-> CHEBI_2509
    ing13_4__Na2CO3["4% Na2CO3"]
    CultureMech_009136 --> ing13_4__Na2CO3
    ing14_10__Glucose["10% Glucose"]
    CultureMech_009136 --> ing14_10__Glucose
    ing15_1__Sodium_fumarate["1% Sodium fumarate"]
    CultureMech_009136 --> ing15_1__Sodium_fumarate
    ing16_0_5__Dithiothreitol__DTT_["0.5% Dithiothreitol (DTT)"]
    CultureMech_009136 --> ing16_0_5__Dithiothreitol__DTT_
    ing17_4__L_Cysteine___HCl["4% L-Cysteine . HCl"]
    CultureMech_009136 --> ing17_4__L_Cysteine___HCl
    ing18_Defibrinated_sheep_blood["Defibrinated sheep blood"]
    CultureMech_009136 --> ing18_Defibrinated_sheep_blood
    ing19_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009136 --> ing19_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing19_Carbon_dioxide_gas -.-> CHEBI_16526
    ing20_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009136 --> ing20_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing20_Nitrogen_gas -.-> CHEBI_17997
    ing21_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009136 --> ing21_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing21_Hydrogen_gas -.-> CHEBI_18276
    ing22_95__Ethanol["95% Ethanol"]
    CultureMech_009136 --> ing22_95__Ethanol
    ing23_Hemin["Hemin"]
    CultureMech_009136 --> ing23_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing23_Hemin -.-> CHEBI_50385
    ing24_KOH["KOH"]
    CultureMech_009136 --> ing24_KOH
    CHEBI_32035["CHEBI:32035"]
    ing24_KOH -.-> CHEBI_32035
    ing25_Menadione__Vitamin_K3_["Menadione (Vitamin K3)"]
    CultureMech_009136 --> ing25_Menadione__Vitamin_K3_

Ingredients (26)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1074.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 3 duplicates: 924.0, 100.0, 50.0]
2	Yeast extract	1 G_PER_L		Role: Nutrient source; Properties: Undefined component
3	KNO3 K.NO3 101.102 g/mol	0.5 G_PER_L	CHEBI:63043 potassium nitrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	Sodium succinate C4H4O4.2Na 162.052 g/mol	0.5 G_PER_L	CHEBI:63675 sodium succinate (anhydrous)	Role: Growth factor; Properties: Simple component, Organic compound, Defined component
5	Sodium lactate, 60% syrup	1.3 G_PER_L		Role: Carbon source; Properties: Defined component, Simple component, Organic compound
6	Agar	4 G_PER_L	CHEBI:2509 agar	Role: Solidifying component
7	Sodium formate CHO2.Na 68.007 g/mol	0.5 G_PER_L	CHEBI:62965 sodium formate	Properties: Defined component, Inorganic compound, Simple component
8	Pancreatic digest of casein Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	17.0 G_PER_L	MICRO:0000182 (source link unavailable) tryptone	Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
9	Peptic digest of soybean meal Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	3.0 G_PER_L	FOODON:03315720 vegetable protein, hydrolyzed	Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
10	Glucose C6H12O6 180.156 g/mol Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	2.5 G_PER_L	CHEBI:17234 glucose	Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
11	Sodium chloride Cl.Na 58.443 g/mol Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	5.0 G_PER_L	CHEBI:26710 sodium chloride	Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
12	Dipotassium phosphate HO4P.2K 174.174 g/mol Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	2.5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
13	Agar Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)	15.0 G_PER_L	CHEBI:2509 agar	Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
14	4% Na2CO3	10 G_PER_L		Role: Buffer; Properties: Defined component, Inorganic compound, Solution
15	10% Glucose	10 G_PER_L		Role: Carbon source; Properties: Defined component, Solution, Organic compound
16	1% Sodium fumarate	2 G_PER_L		Properties: Defined component, Solution, Organic compound
17	0.5% Dithiothreitol (DTT)	10 G_PER_L		Properties: Defined component, Simple component, Inorganic compound
18	4% L-Cysteine . HCl	10 G_PER_L		Properties: Organic compound, Simple component, Defined component
19	Defibrinated sheep blood	30 G_PER_L		Role: Nutrient source; Properties: Undefined component
20	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
21	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
22	Hydrogen gas H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	Properties: ガス
23	95% Ethanol	150.0 G_PER_L		Role: Solvating media; Properties: Organic compound, Simple component, Defined component [Merged 2 duplicates: 100.0, 50.0]
24	Hemin C34H32ClFeN4O4 651.952 g/mol	200 G_PER_L	CHEBI:50385 hemin	Role: Growth factor; Properties: Defined component, Simple component, Organic compound
25	KOH HO.K 56.105 g/mol	1.12 G_PER_L	CHEBI:32035 potassium hydroxide	Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
26	Menadione (Vitamin K3)	50 G_PER_L		Role: Vitamin; Properties: Simple component, Organic compound, Defined component

Solutions

Hemin Solution

Dose: 1 G_PER_L

See mediadive_5229_Hemin_solution.yaml for composition

Menadione Solution

Dose: 2 G_PER_L

See mediadive_5549_Menadione_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.758149Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2568
2026-01-28T19:48:29.792617Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:49.499134+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 2 solution(s) from ingredients
2026-03-13T03:09:20.650269Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 3 duplicate ingredient(s)
2026-03-13T06:20:03.155615Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009136
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name
2026-03-17T06:30:28.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:21.890445+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ETSA medium" to "etsa_medium" for consistent matching
2026-05-16T09:03:31.565955+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:59.702505+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.