Overview
- External ontology
- TOGO:M2569 — Dictyoglomus medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2569 Original URL: https://www.atcc.org/~/media/BCDDD7524B3B4F88B1BDE51C6EFDBB75.ashx
Composition
flowchart LR
CultureMech_009137["dictyoglomus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009137 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009137 --> ing1_Yeast_extract
ing2_KH2PO4["KH2PO4"]
CultureMech_009137 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_009137 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Resazurin["Resazurin"]
CultureMech_009137 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MgCl2___6H2O["MgCl2 . 6H2O"]
CultureMech_009137 --> ing5_MgCl2___6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2___6H2O -.-> CHEBI_86345
ing6_Na2CO3["Na2CO3"]
CultureMech_009137 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_CaCl2["CaCl2"]
CultureMech_009137 --> ing7_CaCl2
CHEBI_3312["CHEBI:3312"]
ing7_CaCl2 -.-> CHEBI_3312
ing8_Fe_NH4_2_SO4_2___6H2O["Fe(NH4)2(SO4)2 . 6H2O"]
CultureMech_009137 --> ing8_Fe_NH4_2_SO4_2___6H2O
CHEBI_76181["CHEBI:76181"]
ing8_Fe_NH4_2_SO4_2___6H2O -.-> CHEBI_76181
ing9_Na2HPO4___12H2O["Na2HPO4 . 12H2O"]
CultureMech_009137 --> ing9_Na2HPO4___12H2O
CHEBI_34683["CHEBI:34683"]
ing9_Na2HPO4___12H2O -.-> CHEBI_34683
ing10_Soluble_starch["Soluble starch"]
CultureMech_009137 --> ing10_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing10_Soluble_starch -.-> CHEBI_28017
ing11_Polypeptone_Peptone__BD_211910_["Polypeptone Peptone (BD 211910)"]
CultureMech_009137 --> ing11_Polypeptone_Peptone__BD_211910_
ing12_L_Cysteine___HCl["L-Cysteine . HCl"]
CultureMech_009137 --> ing12_L_Cysteine___HCl
ing13_Trace_Metals["Trace Metals"]
CultureMech_009137 --> ing13_Trace_Metals
ing14_Nitrogen["Nitrogen"]
CultureMech_009137 --> ing14_Nitrogen
CHEBI_17997["CHEBI:17997"]
ing14_Nitrogen -.-> CHEBI_17997
ing15_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009137 --> ing15_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4___2H2O -.-> CHEBI_75213
ing16_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009137 --> ing16_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2___4H2O -.-> CHEBI_86368
ing17_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009137 --> ing17_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing17_CoCl2___6H2O -.-> CHEBI_35696
ing18_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009137 --> ing18_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4___7H2O -.-> CHEBI_32312
ing19_Na2SeO3["Na2SeO3"]
CultureMech_009137 --> ing19_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing19_Na2SeO3 -.-> CHEBI_48843
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
981.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 980.0, 1.0] |
| 2 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
2 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
MgCl2 . 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.38 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.05 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Fe(NH4)2(SO4)2 . 6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.039 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2HPO4 . 12H2O
HO4P.2Na 141.958 g/mol
|
4.2 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Soluble starch | 5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 12 | Polypeptone Peptone (BD 211910) | 2 G_PER_L | Properties: Undefined component | |
| 13 | L-Cysteine . HCl | 1 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 14 | Trace Metals | 10 G_PER_L | ||
| 15 |
Nitrogen
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 16 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
240 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
200 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
290 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
280 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
17 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Wolfe's Vitamin Solution
Dose: 10 G_PER_L
Role: Growth factor; Properties: Complex component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.760971Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2569 |
| 2026-01-28T19:48:37.263770Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen |
| 2026-03-09T08:05:02.161344+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:34.029870Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:03.167095Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009137 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.901544+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Dictyoglomus medium" to "dictyoglomus_medium" for consistent matching |
| 2026-05-16T09:03:14.269211+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.527765+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.278017+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |