Overview
- External ontology
- TOGO:M2570 — Fibrobacter medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2570 Original URL: https://www.atcc.org/~/media/0FCD78F660A9430BBD47DAA50E91D276.ashx Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009139["fibrobacter_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009139 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009139 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009139 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009139 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009139 --> ing4_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2___2H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_009139 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_K2HPO4["K2HPO4"]
CultureMech_009139 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Resazurin["Resazurin"]
CultureMech_009139 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_009139 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_Na2CO3["Na2CO3"]
CultureMech_009139 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_Cellobiose["Cellobiose"]
CultureMech_009139 --> ing10_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing10_Cellobiose -.-> CHEBI_17057
ing11_Tryptone__BD_211705_["Tryptone (BD 211705)"]
CultureMech_009139 --> ing11_Tryptone__BD_211705_
ing12_CO2["CO2"]
CultureMech_009139 --> ing12_CO2
CHEBI_16526["CHEBI:16526"]
ing12_CO2 -.-> CHEBI_16526
ing13_2_5__Na2S___9H2O["2.5% Na2S . 9H2O"]
CultureMech_009139 --> ing13_2_5__Na2S___9H2O
ing14_2_5__L_Cysteine___HCl["2.5% L-Cysteine . HCl"]
CultureMech_009139 --> ing14_2_5__L_Cysteine___HCl
ing15_Nitrogen["Nitrogen"]
CultureMech_009139 --> ing15_Nitrogen
CHEBI_17997["CHEBI:17997"]
ing15_Nitrogen -.-> CHEBI_17997
ing16_Biotin["Biotin"]
CultureMech_009139 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009139 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18_Thiamine___HCl["Thiamine . HCl"]
CultureMech_009139 --> ing18_Thiamine___HCl
ing19_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_009139 --> ing19_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing19_Calcium_D_____pantothenate -.-> CHEBI_31345
ing20_Riboflavin["Riboflavin"]
CultureMech_009139 --> ing20_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing20_Riboflavin -.-> CHEBI_17015
ing21_Nicotinamide["Nicotinamide"]
CultureMech_009139 --> ing21_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing21_Nicotinamide -.-> CHEBI_17154
ing22_Pyridoxal_hydrochloride["Pyridoxal hydrochloride"]
CultureMech_009139 --> ing22_Pyridoxal_hydrochloride
CHEBI_131529["CHEBI:131529"]
ing22_Pyridoxal_hydrochloride -.-> CHEBI_131529
ing23_Pyridoxamine___2HCl["Pyridoxamine . 2HCl"]
CultureMech_009139 --> ing23_Pyridoxamine___2HCl
ing24_Lipoic_acid["Lipoic acid"]
CultureMech_009139 --> ing24_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing24_Lipoic_acid -.-> CHEBI_16494
ing25_Cyanocobalamin["Cyanocobalamin"]
CultureMech_009139 --> ing25_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing25_Cyanocobalamin -.-> CHEBI_17439
ing26_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009139 --> ing26_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing26_Na2MoO4___2H2O -.-> CHEBI_75213
ing27_H3BO3["H3BO3"]
CultureMech_009139 --> ing27_H3BO3
CHEBI_33118["CHEBI:33118"]
ing27_H3BO3 -.-> CHEBI_33118
ing28_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009139 --> ing28_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing28_FeSO4___7H2O -.-> CHEBI_75836
ing29_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009139 --> ing29_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing29_MnCl2___4H2O -.-> CHEBI_86368
CultureMech_009139 --> more["...14 more"]
Ingredients (44)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1371.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 960.0, 100.0, 1.0, 310.0] |
| 2 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.12 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.6 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.08 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Cellobiose
C12H22O11 342.297 g/mol
|
4 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 12 | Tryptone (BD 211705) | 1 G_PER_L | Properties: Undefined component | |
| 13 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 14 | 2.5% Na2S . 9H2O | 10 G_PER_L | Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution | |
| 15 | 2.5% L-Cysteine . HCl | 10 G_PER_L | ||
| 16 |
Nitrogen
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
1 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 | Thiamine . HCl | 20 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 20 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
20 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
20 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
20 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 23 |
Pyridoxal hydrochloride
C8H9NO3.HCl 203.625 g/mol
|
20 G_PER_L |
CHEBI:131529
pyridoxal hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 | Pyridoxamine . 2HCl | 20 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 25 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
20 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 26 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
1 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin |
| 27 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
H3BO3
H3BO3 61.833 g/mol
|
0.2 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 35 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 36 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 37 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 38 |
n-Butyric acid
C4H8O2 88.106 g/mol
|
4 G_PER_L |
CHEBI:30772
butyric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 39 |
Acetic acid
C2H4O2 60.052 g/mol
|
17 G_PER_L |
CHEBI:15366
acetic acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 40 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:17418
valeric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 41 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
1 G_PER_L |
CHEBI:28484
isovaleric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 42 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
1 G_PER_L |
CHEBI:16135
isobutyric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 43 |
Propionic acid
C3H6O2 74.079 g/mol
|
6 G_PER_L |
CHEBI:30768
propionic acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 44 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
Solutions
Vitamin Solution
Dose: 20 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Trace Elements
Dose: 1 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
VFA Solution
Dose: 4.65 G_PER_L
See mediadive_4622_VFA_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.763598Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2570 |
| 2026-01-28T19:48:16.859888Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: Nitrogen; Added default concentration for ingredient: NaOH |
| 2026-02-06T05:10:24.149311Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:27.856496+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:56.617103Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:03.193139Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009139 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.926297+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Fibrobacter medium" to "fibrobacter_medium" for consistent matching |
| 2026-05-16T09:03:31.991674+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:00.366497+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:43.582504+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:56.863011+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |