Overview
- External ontology
- TOGO:M2577 — Modified NOS Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2577 Original URL: https://www.atcc.org/~/media/C14CA5B6B5D246A6B8073D6894BFE5EB.ashx Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009146["modified_nos_medium"]
ing0_D_Glucose["D-Glucose"]
CultureMech_009146 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Resazurin__0_1__w_v_in_water_["Resazurin (0.1% w/v in water)"]
CultureMech_009146 --> ing1_Resazurin__0_1__w_v_in_water_
ing2_DI_Water["DI Water"]
CultureMech_009146 --> ing2_DI_Water
ing3_L_Asparagine["L-Asparagine"]
CultureMech_009146 --> ing3_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing3_L_Asparagine -.-> CHEBI_17196
ing4_Yeast_Extract__BD_211928_["Yeast Extract (BD 211928)"]
CultureMech_009146 --> ing4_Yeast_Extract__BD_211928_
ing5_Trypticase_Peptone__BD_211921_["Trypticase Peptone (BD 211921)"]
CultureMech_009146 --> ing5_Trypticase_Peptone__BD_211921_
ing6_Sodium_thioglycollate["Sodium thioglycollate"]
CultureMech_009146 --> ing6_Sodium_thioglycollate
CHEBI_86481["CHEBI:86481"]
ing6_Sodium_thioglycollate -.-> CHEBI_86481
ing7_Heart_Infusion_Broth__BD_238400_["Heart Infusion Broth (BD 238400)"]
CultureMech_009146 --> ing7_Heart_Infusion_Broth__BD_238400_
ing8_L_Cysteine_x_HCl["L-Cysteine x HCl"]
CultureMech_009146 --> ing8_L_Cysteine_x_HCl
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009146 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Nitrogen_gas["Nitrogen gas"]
CultureMech_009146 --> ing10_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing10_Nitrogen_gas -.-> CHEBI_17997
ing11_Hydrogen_gas["Hydrogen gas"]
CultureMech_009146 --> ing11_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing11_Hydrogen_gas -.-> CHEBI_18276
ing12_NaHCO3__7_5__w_v_in_water_["NaHCO3 (7.5% w/v in water)"]
CultureMech_009146 --> ing12_NaHCO3__7_5__w_v_in_water_
ing13_Rabbit_serum["Rabbit serum"]
CultureMech_009146 --> ing13_Rabbit_serum
ing14_Thiamine_pyrophosphate__0_2__w_v_in_water_["Thiamine pyrophosphate (0.2% w/v in water)"]
CultureMech_009146 --> ing14_Thiamine_pyrophosphate__0_2__w_v_in_water_
ing15_0_1_N_NaOH["0.1 N NaOH"]
CultureMech_009146 --> ing15_0_1_N_NaOH
ing16_Valeric_acid["Valeric acid"]
CultureMech_009146 --> ing16_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing16_Valeric_acid -.-> CHEBI_17418
ing17_Isovaleric_acid["Isovaleric acid"]
CultureMech_009146 --> ing17_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing17_Isovaleric_acid -.-> CHEBI_28484
ing18_Isobutyric_acid["Isobutyric acid"]
CultureMech_009146 --> ing18_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing18_Isobutyric_acid -.-> CHEBI_16135
ing19_Tryptone["Tryptone"]
CultureMech_009146 --> ing19_Tryptone
ing20_Yeast_extract["Yeast extract"]
CultureMech_009146 --> ing20_Yeast_extract
ing21_Sodium_chloride["Sodium chloride"]
CultureMech_009146 --> ing21_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing21_Sodium_chloride -.-> CHEBI_26710
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
0.2 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 2 | Resazurin (0.1% w/v in water) | 0.1 G_PER_L | Role: pH dependent redox indicator; Properties: Solution, Defined component | |
| 3 | DI Water | 102.4 G_PER_L | Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 94.0, 8.4] | |
| 4 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
0.025 G_PER_L |
CHEBI:17196
L-asparagine
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 5 | Yeast Extract (BD 211928) | 0.25 G_PER_L | Properties: Undefined component | |
| 6 | Trypticase Peptone (BD 211921) | 1 G_PER_L | Properties: Undefined component | |
| 7 |
Sodium thioglycollate
C2H3O2S.Na 114.101 g/mol
|
0.05 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Organic compound, Simple component |
| 8 | Heart Infusion Broth (BD 238400) | 1.25 G_PER_L | Properties: Complex component, Undefined component | |
| 9 | L-Cysteine x HCl | 0.1 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 13 | NaHCO3 (7.5% w/v in water) | 2.67 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Inorganic compound | |
| 14 | Rabbit serum | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 15 | Thiamine pyrophosphate (0.2% w/v in water) | 1.5 G_PER_L | Role: Vitamin; Properties: Solution, Organic compound, Defined component | |
| 16 | 0.1 N NaOH | 100 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 17 |
Valeric acid
C5H10O2 102.133 g/mol
|
0.5 G_PER_L |
CHEBI:17418
valeric acid
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 18 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
0.5 G_PER_L |
CHEBI:28484
isovaleric acid
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 19 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
0.5 G_PER_L |
CHEBI:16135
isobutyric acid
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 20 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 21 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 22 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Solutions
TPP/VFA Solution
Dose: 2 G_PER_L
VFA Solution
Dose: 1 G_PER_L
See mediadive_4622_VFA_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.781335Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2577 |
| 2026-01-28T19:48:36.100427Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:05:00.192002+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:32.015674Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:03.261525Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009146 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.991441+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified NOS Medium" to "modified_nos_medium" for consistent matching |
| 2026-05-16T09:03:14.307023+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.570053+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |