Overview
- External ontology
- TOGO:M257 — Methanogenium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M257 Original source: JCM - JCM_M265 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=265 Post-inoculation source instruction: Pressurize the inoculated bottles to 200 kPa H2:CO2 (80:20, v/v).
Composition
flowchart LR
CultureMech_009148["methanogenium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009148 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009148 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009148 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009148 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009148 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009148 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_009148 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009148 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_009148 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaHCO3["NaHCO3"]
CultureMech_009148 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Sodium_acetate["Sodium acetate"]
CultureMech_009148 --> ing10_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing10_Sodium_acetate -.-> CHEBI_32954
ing11_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2・6H2O"]
CultureMech_009148 --> ing11_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing11_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009148 --> ing12_Yeast_extract__BD_Difco_
ing13_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_009148 --> ing13_Trypticase_peptone__BD_BBL_
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009148 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
ing15_N2["N2"]
CultureMech_009148 --> ing15_N2
CHEBI_17997["CHEBI:17997"]
ing15_N2 -.-> CHEBI_17997
ing16_Hydrogen_gas["Hydrogen gas"]
CultureMech_009148 --> ing16_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing16_Hydrogen_gas -.-> CHEBI_18276
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
0.98 L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component Water batch input: 980 ml / 1000 ml per L = 0.98 L, not a measured final volume. |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 3.45 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 18 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.14 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.14 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.25 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component Source batch input: 1 mg. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 4 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 9 |
KCl
Cl.K 74.551 g/mol
|
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.355 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Source batch input: 5 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 11 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component Source batch input: 1 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 12 |
Fe(NH4)2(SO4)2・6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 2 mg. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| 13 | Yeast extract (BD-Difco) | Properties: Undefined component Source batch input: 2 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | ||
| 14 | Trypticase peptone (BD-BBL) | Properties: Undefined component Source batch input: 2 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | ||
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 16 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 17 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Preparation
- Mix components thoroughly, except NaHCO3, and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of H2--CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 5 ml medium in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
Solutions
Wolfe's mineral solution (see below)
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| NiCl2・6H2O | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.02 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| Na2WO4・2H2O | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.01 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. | |
| Na2SeO3 | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Source batch input: 0.001 g. The former g/L assertion is withdrawn because final preparation volume is not independently established. Evidence supports this batch-amount note, not a calculated concentration. |
Source batch stock input: 10 ml = 0.01 L, not a dose per final liter. Stock composition, where given, belongs to this preparation and is not a direct receiving-medium ingredient list.
Trace minerals (see Medium [M142])
Dose: 1 L
Cross-reference to Medium M142
Source batch stock input: 1 L = 1 L, not a dose per final liter. Stock composition, where given, belongs to this preparation and is not a direct receiving-medium ingredient list.
Trace vitamins (see Medium [M190])
Dose: 0.01 L
Cross-reference to Medium M190
Source batch stock input: 10 ml = 0.01 L, not a dose per final liter. Stock composition, where given, belongs to this preparation and is not a direct receiving-medium ingredient list.
5% Na2S・9H2O solution
Dose: 10 ML_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
The preparation explicitly prescribes 10 ml per liter at this stage, not 10 g/L or an absolute batch dose. Stock composition, where given, belongs to this preparation and is not a direct receiving-medium ingredient list.
Source 5% reagent label has unspecified percentage basis. The asserted numeric value is its per-liter addition volume only, not its mass concentration or an inferred stock strength.
5% L-Cysteine・HCl・H2O solution
Dose: 10 ML_PER_L
Properties: Defined component, Organic compound, Solution
The preparation explicitly prescribes 10 ml per liter at this stage, not 10 g/L or an absolute batch dose. Stock composition, where given, belongs to this preparation and is not a direct receiving-medium ingredient list.
Source 5% reagent label has unspecified percentage basis. The asserted numeric value is its per-liter addition volume only, not its mass concentration or an inferred stock strength.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.711489Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M257 |
| 2026-01-28T19:48:25.350819Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:41.943711+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T06:20:03.281727Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009148 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.011443+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanogenium Medium" to "methanogenium_medium" for consistent matching |
| 2026-05-16T09:03:14.321486+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.584649+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.253282+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.287073+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T13:12:36.691952+00:00 | codex | RESTORED_METHANOGEN_STOCK_BOUNDARIES_AND_DOSE_BASES_WITH_EVIDENCE | Restored preparation boundaries and explicit stock-dose bases. Preserved source-specific conflicts, chemical annotations and earlier history. Batch-mass notes do not assert final concentrations; provider-declared stock rates remain qualified. No growth or identity equivalence claim inferred. |