Overview
- External ontology
- TOGO:M2583 — Mueller Hinton Medium with 10% Rabbit Serum
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2583 records 22 g BD 211443 Mueller Hinton Broth in 900 ml DI Water, autoclaved at 121 C, cooled, and aseptically supplemented with 100 ml sterile rabbit serum.
Composition
flowchart LR
CultureMech_009151["mueller_hinton_medium_with_10_rabbit_serum"]
ing0_DI_Water["DI Water"]
CultureMech_009151 --> ing0_DI_Water
CHEBI_15377["CHEBI:15377"]
ing0_DI_Water -.-> CHEBI_15377
ing1_Mueller_Hinton_Broth__BD_211443_["Mueller Hinton Broth (BD 211443)"]
CultureMech_009151 --> ing1_Mueller_Hinton_Broth__BD_211443_
ing2_Rabbit_serum["Rabbit serum"]
CultureMech_009151 --> ing2_Rabbit_serum
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
DI Water
H2O 18.015 g/mol
|
900 ML_PER_L |
CHEBI:15377
water
|
TOGO M2583 lists 900 ml DI Water. |
| 2 | Mueller Hinton Broth (BD 211443) | 22 G_PER_L | TOGO M2583 lists 22 g Mueller Hinton Broth from BD catalog 211443 without disclosing the broth composition. | |
| 3 | Rabbit serum | 100 ML_PER_L | TOGO M2583 says to aseptically add 100 ml of sterile rabbit serum. |
Preparation
- Prepare 900 ml DI Water with 22 g Mueller Hinton Broth (BD 211443).
- Autoclave at 121 C and let cool.
- Aseptically add 100 ml sterile rabbit serum.
- Dispense into an appropriate vessel.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.787523Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2583 |
| 2026-03-13T06:20:03.294847Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009151 |
| 2026-03-17T19:46:22.023765+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mueller Hinton Medium with 10% Rabbit Serum" to "mueller_hinton_medium_with_10_rabbit_serum" for consistent matching |
| 2026-09-10T00:00:00-07:00 | repair_togo_m2583_score25.py | RESOLVED_TOGO_M2583_SCORE25 | TOGO M2583 records 22 g BD 211443 Mueller Hinton Broth in 900 ml DI Water, autoclaved at 121 C, cooled, and aseptically supplemented with 100 ml sterile rabbit serum. |