Overview
- External ontology
- TOGO:M2591 — Flexibacter Canadensis Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2591 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium357.pdf
Composition
flowchart LR
CultureMech_009158["flexibacter_canadensis_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009158 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009158 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009158 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_Vitamin_B12["Vitamin B12"]
CultureMech_009158 --> ing3_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing3_Vitamin_B12 -.-> CHEBI_176843
ing4_KNO3["KNO3"]
CultureMech_009158 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_Tris["Tris"]
CultureMech_009158 --> ing5_Tris
CHEBI_9754["CHEBI:9754"]
ing5_Tris -.-> CHEBI_9754
ing6_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_009158 --> ing6_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing6_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing7_Na_glycerophosphate["Na-glycerophosphate"]
CultureMech_009158 --> ing7_Na_glycerophosphate
ing8_Glucose__sterilized_separately_["Glucose (sterilized separately)"]
CultureMech_009158 --> ing8_Glucose__sterilized_separately_
ing9_Agar["Agar"]
CultureMech_009158 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Casamino_acids["Casamino acids"]
CultureMech_009158 --> ing10_Casamino_acids
ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009158 --> ing11_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_009158 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009158 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009158 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009158 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009158 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_ZnCl2["ZnCl2"]
CultureMech_009158 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
CultureMech_009158 --> ing18_HCl__25___7_7_M_
ing19_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_009158 --> ing19_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing19_FeCl2_x_4_H2O -.-> CHEBI_86249
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1990.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1000.0, 990.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 5 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Tris
C4H11NO3 121.136 g/mol
|
1 G_PER_L |
CHEBI:9754
tris
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 7 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
1 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 8 | Na-glycerophosphate | 0.1 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 9 | Glucose (sterilized separately) | 1 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component | |
| 10 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 11 | Casamino acids | 1 G_PER_L | Properties: Undefined component | |
| 12 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
190 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 | HCl (25%; 7.7 M) | 10 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Solution | |
| 20 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution SL-10
Dose: 1 G_PER_L
See mediadive_4178_Trace_element_solution_SL-10.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.800760Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2591 |
| 2026-01-28T19:48:37.200254Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:02.067129+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:33.935996Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:03.373738Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009158 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.101596+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Flexibacter Canadensis Medium" to "flexibacter_canadensis_medium" for consistent matching |
| 2026-05-16T09:03:14.418286+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:37.684982+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.746877+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:27.340595+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.320699+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |