Overview
- External ontology
- TOGO:M2593 — K-101 Flexibacter medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2593 Original URL: https://www.atcc.org/~/media/8FF72E559CC4471D97C7CF6AAD2E86CE.ashx
Composition
flowchart LR
CultureMech_009160["k_101_flexibacter_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009160 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009160 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Thiamine___HCl["Thiamine . HCl"]
CultureMech_009160 --> ing2_Thiamine___HCl
ing3_Vitamin_B12["Vitamin B12"]
CultureMech_009160 --> ing3_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing3_Vitamin_B12 -.-> CHEBI_176843
ing4_KNO3["KNO3"]
CultureMech_009160 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_CaCl2["CaCl2"]
CultureMech_009160 --> ing5_CaCl2
CHEBI_3312["CHEBI:3312"]
ing5_CaCl2 -.-> CHEBI_3312
ing6_Sodium_glycerophosphate["Sodium glycerophosphate"]
CultureMech_009160 --> ing6_Sodium_glycerophosphate
ing7_Tris_buffer["Tris buffer"]
CultureMech_009160 --> ing7_Tris_buffer
CHEBI_9754["CHEBI:9754"]
ing7_Tris_buffer -.-> CHEBI_9754
ing8_Glucose["Glucose"]
CultureMech_009160 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Agar["Agar"]
CultureMech_009160 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Casamino_acids["Casamino acids"]
CultureMech_009160 --> ing10_Casamino_acids
ing11_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009160 --> ing11_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4___2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_009160 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009160 --> ing13_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2___4H2O -.-> CHEBI_86368
ing14_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009160 --> ing14_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing14_CoCl2___6H2O -.-> CHEBI_35696
ing15_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009160 --> ing15_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2___2H2O -.-> CHEBI_86318
ing16_ZnCl2["ZnCl2"]
CultureMech_009160 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_Sodium_tartrate["Sodium tartrate"]
CultureMech_009160 --> ing17_Sodium_tartrate
CHEBI_63017["CHEBI:63017"]
ing17_Sodium_tartrate -.-> CHEBI_63017
ing18_FeSO4_["FeSO4 "]
CultureMech_009160 --> ing18_FeSO4_
CHEBI_75832["CHEBI:75832"]
ing18_FeSO4_ -.-> CHEBI_75832
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Thiamine . HCl | 1 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 4 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 5 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Sodium glycerophosphate | 0.1 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 8 |
Tris buffer
C4H11NO3 121.136 g/mol
|
1 G_PER_L |
CHEBI:9754
tris
|
Role: Buffer; Properties: Defined component, Complex component, Solution |
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 10 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 11 | Casamino acids | 1 G_PER_L | Properties: Undefined component | |
| 12 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
25.2 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
2.85 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
40.4 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
26.9 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
20.8 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Sodium tartrate
C4H4O6.2Na 194.05 g/mol
|
1.77 G_PER_L |
CHEBI:63017
sodium L-tartrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 19 |
FeSO4
Fe.O4S 151.908 g/mol
|
1.36 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace Elements
Dose: 1 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.804806Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2593 |
| 2026-01-28T19:48:16.690548Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:27.575776+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:56.318149Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:03.391897Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009160 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.120065+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "K-101 Flexibacter medium" to "k_101_flexibacter_medium" for consistent matching |
| 2026-05-16T09:03:38.402582+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:10.274624+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.250552+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:49.992181+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.335286+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:11.821521+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |