Overview
- External ontology
- TOGO:M2595 — Pfennig's Medium II
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2595 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium29.pdf
Composition
flowchart LR
CultureMech_009162["pfennigs_medium_ii"]
ing0_1M_Na2CO3["1M Na2CO3"]
CultureMech_009162 --> ing0_1M_Na2CO3
ing1_N2_gas["N2 gas"]
CultureMech_009162 --> ing1_N2_gas
ing2_CO2["CO2"]
CultureMech_009162 --> ing2_CO2
CHEBI_16526["CHEBI:16526"]
ing2_CO2 -.-> CHEBI_16526
ing3_Distilled_water["Distilled water"]
CultureMech_009162 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_Yeast_extrakt["Yeast extrakt"]
CultureMech_009162 --> ing4_Yeast_extrakt
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009162 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009162 --> ing6_Carbon_dioxide_gas
ing6_Carbon_dioxide_gas -.-> CHEBI_16526
ing7_Nitrogen_gas["Nitrogen gas"]
CultureMech_009162 --> ing7_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing7_Nitrogen_gas -.-> CHEBI_17997
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009162 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_N2["N2"]
CultureMech_009162 --> ing9_N2
ing9_N2 -.-> CHEBI_17997
ing10_H2O["H2O"]
CultureMech_009162 --> ing10_H2O
ing10_H2O -.-> CHEBI_15377
ing11_NaHCO3["NaHCO3"]
CultureMech_009162 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Resazurin__0_1__["Resazurin (0,1%)"]
CultureMech_009162 --> ing12_Resazurin__0_1__
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009162 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_KH2PO4["KH2PO4"]
CultureMech_009162 --> ing14_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing14_KH2PO4 -.-> CHEBI_63036
ing15_Ammonium_chloride["Ammonium chloride"]
CultureMech_009162 --> ing15_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing15_Ammonium_chloride -.-> CHEBI_31206
ing16_Dextrose["Dextrose"]
CultureMech_009162 --> ing16_Dextrose
CHEBI_17634["CHEBI:17634"]
ing16_Dextrose -.-> CHEBI_17634
ing17_KCL["KCL"]
CultureMech_009162 --> ing17_KCL
CHEBI_32588["CHEBI:32588"]
ing17_KCL -.-> CHEBI_32588
ing18_Pyruvic_acid_sodium_salt["Pyruvic acid sodium salt"]
CultureMech_009162 --> ing18_Pyruvic_acid_sodium_salt
CHEBI_50144["CHEBI:50144"]
ing18_Pyruvic_acid_sodium_salt -.-> CHEBI_50144
ing19_Ammonium_acetate["Ammonium acetate"]
CultureMech_009162 --> ing19_Ammonium_acetate
CHEBI_62947["CHEBI:62947"]
ing19_Ammonium_acetate -.-> CHEBI_62947
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_009162 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_HCl__25__["HCl (25%)"]
CultureMech_009162 --> ing21_HCl__25__
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009162 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_H3BO3["H3BO3"]
CultureMech_009162 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009162 --> ing24_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
ing25_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009162 --> ing25_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing25_MnCl2_x_4_H2O -.-> CHEBI_86368
ing26_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009162 --> ing26_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing26_CoCl2_x_6_H2O -.-> CHEBI_53503
ing27_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009162 --> ing27_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing27_NiCl2_x_6_H2O -.-> CHEBI_34887
ing28_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009162 --> ing28_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing28_CuCl2_x_2_H2O -.-> CHEBI_86318
ing29_ZnCl2["ZnCl2"]
CultureMech_009162 --> ing29_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing29_ZnCl2 -.-> CHEBI_49976
CultureMech_009162 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 1M Na2CO3 | variable VARIABLE | Role: Buffer; Properties: Defined component, Inorganic compound, Solution | |
| 2 | N2 gas | variable VARIABLE | Properties: ガス | |
| 3 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 4 |
Distilled water
H2O 18.015 g/mol
|
2270.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 460.0, 135.0, 450.0, 25.0, 100.0, 1000.0, 100.0] |
| 5 | Yeast extrakt | 0.25 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 8 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
5.0 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.0, 3.0] |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 |
H2O
H2O 18.015 g/mol
|
50 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 13 | Resazurin (0,1%) | 0.5 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.35 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
0.35 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Dextrose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 18 |
KCL
Cl.K 74.551 g/mol
|
0.35 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Pyruvic acid sodium salt
C3H3O3.Na 110.044 g/mol
|
0.25 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 20 |
Ammonium acetate
C2H3O2.H4N 77.083 g/mol
|
0.25 G_PER_L |
CHEBI:62947
ammonium acetate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 21 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 | HCl (25%) | 7.7 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
300 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
190 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 | 2N H2SO4 | variable VARIABLE | Role: Mineral source; Properties: Defined component, Solution, Inorganic compound |
Solutions
Solution A
Dose: 46 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution D+C+E
Dose: 50 G_PER_L
Solution B
Dose: 4 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution F
Dose: 0.1 G_PER_L
See mediadive_6293_Solution_F.yaml for composition
Neutralized sulfide solution
Dose: variable VARIABLE
Solution D
Dose: 450.5 G_PER_L
See mediadive_5313_Solution_D.yaml for composition
Solution C
Dose: 50 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Solution E
Dose: 26 G_PER_L
See mediadive_4959_Solution_E.yaml for composition
Trace element solution SL-10 B
Dose: 1 G_PER_L
See mediadive_2396_Trace_element_solution_SL-10_B.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.812675Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2595 |
| 2026-01-28T19:48:36.132361Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1M Na2CO3; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: Neutralized sulfide solution; Added default concentration for ingredient: CO2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: CO2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: 2N H2SO4; Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:00.252929+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 9 solution(s) from ingredients |
| 2026-03-13T03:09:32.084708Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 11 duplicate ingredient(s) |
| 2026-03-13T06:20:03.434280Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009162 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.161819+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pfennig's Medium II" to "pfennigs_medium_ii" for consistent matching |
| 2026-05-16T09:03:14.469024+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.736885+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.339479+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |