Overview
- External ontology
- TOGO:M2598 — Methanogenium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2598 Original URL: https://www.atcc.org/~/media/78EFFF1EF0A641FD9B9EADC81C711704.ashx
Composition
flowchart LR
CultureMech_009165["methanogenium_medium"]
ing0_MgSO4_x_7H2O["MgSO4 x 7H2O"]
CultureMech_009165 --> ing0_MgSO4_x_7H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7H2O -.-> CHEBI_31795
ing1_Yeast_Extract["Yeast Extract"]
CultureMech_009165 --> ing1_Yeast_Extract
ing2_NaCl["NaCl"]
CultureMech_009165 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_x_2H2O["CaCl2 x 2H2O"]
CultureMech_009165 --> ing3_CaCl2_x_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_009165 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_009165 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009165 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Na2S_x_9H2O["Na2S x 9H2O"]
CultureMech_009165 --> ing7_Na2S_x_9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9H2O -.-> CHEBI_76209
ing8_KCl["KCl"]
CultureMech_009165 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaHCO3["NaHCO3"]
CultureMech_009165 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Na_Acetate["Na Acetate"]
CultureMech_009165 --> ing10_Na_Acetate
CHEBI_32954["CHEBI:32954"]
ing10_Na_Acetate -.-> CHEBI_32954
ing11_Fe_NH4_2_SO4_2_x_7H2O["Fe(NH4)2(SO4)2 x 7H2O"]
CultureMech_009165 --> ing11_Fe_NH4_2_SO4_2_x_7H2O
CHEBI_131378["CHEBI:131378"]
ing11_Fe_NH4_2_SO4_2_x_7H2O -.-> CHEBI_131378
ing12_MgCl2_x_7H2O["MgCl2 x 7H2O"]
CultureMech_009165 --> ing12_MgCl2_x_7H2O
CHEBI_6636["CHEBI:6636"]
ing12_MgCl2_x_7H2O -.-> CHEBI_6636
ing13_DI_Water["DI Water"]
CultureMech_009165 --> ing13_DI_Water
ing14_Cysteine_HCl_x_H2O["Cysteine HCl x H2O"]
CultureMech_009165 --> ing14_Cysteine_HCl_x_H2O
ing15_Trypticase["Trypticase"]
CultureMech_009165 --> ing15_Trypticase
ing16_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009165 --> ing16_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing16_Carbon_dioxide_gas -.-> CHEBI_16526
ing17_Nitrogen_gas["Nitrogen gas"]
CultureMech_009165 --> ing17_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing17_Nitrogen_gas -.-> CHEBI_17997
ing18_Biotin["Biotin"]
CultureMech_009165 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_p_Aminobenzoic_Acid["p-Aminobenzoic Acid"]
CultureMech_009165 --> ing19_p_Aminobenzoic_Acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_Acid -.-> CHEBI_30753
ing20_Thiamine_x_HCl["Thiamine x HCl"]
CultureMech_009165 --> ing20_Thiamine_x_HCl
ing21_Ca_pantothenate["Ca pantothenate"]
CultureMech_009165 --> ing21_Ca_pantothenate
ing22_Pyridoxine_x_HCl["Pyridoxine x HCl"]
CultureMech_009165 --> ing22_Pyridoxine_x_HCl
ing23_Folic_Acid["Folic Acid"]
CultureMech_009165 --> ing23_Folic_Acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_Acid -.-> CHEBI_27470
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_009165 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_Riboflavin["Riboflavin"]
CultureMech_009165 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Nicotinic_Acid["Nicotinic Acid"]
CultureMech_009165 --> ing26_Nicotinic_Acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_Acid -.-> CHEBI_15940
ing27_Thioctic_Acid["Thioctic Acid"]
CultureMech_009165 --> ing27_Thioctic_Acid
CHEBI_30314["CHEBI:30314"]
ing27_Thioctic_Acid -.-> CHEBI_30314
ing28_Na2MoO4_x_2H2O["Na2MoO4 x 2H2O"]
CultureMech_009165 --> ing28_Na2MoO4_x_2H2O
CHEBI_75213["CHEBI:75213"]
ing28_Na2MoO4_x_2H2O -.-> CHEBI_75213
ing29_H3BO3["H3BO3"]
CultureMech_009165 --> ing29_H3BO3
CHEBI_33118["CHEBI:33118"]
ing29_H3BO3 -.-> CHEBI_33118
CultureMech_009165 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.45 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 2 | Yeast Extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
18 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2 x 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
Na2S x 9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.335 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Na Acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 12 |
Fe(NH4)2(SO4)2 x 7H2O
Fe.7H2O.2H4N.2O4S 410.154 g/mol
|
2 G_PER_L |
CHEBI:131378
ferrous ammonium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MgCl2 x 7H2O
2Cl.Mg 95.211 g/mol
|
2.75 G_PER_L |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | DI Water | 1000.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1000.0, 1000.0, 1000.0] | |
| 15 | Cysteine HCl x H2O | 0.5 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 16 | Trypticase | 2 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
Properties: Undefined component |
| 17 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 18 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
p-Aminobenzoic Acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 | Thiamine x HCl | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 22 | Ca pantothenate | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 23 | Pyridoxine x HCl | 10 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 24 |
Folic Acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Nicotinic Acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Thioctic Acid
C8H14O2S2 206.332 g/mol
|
1 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 29 |
Na2MoO4 x 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
MnCl2 x 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
CoCl2 x 6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
NiCl2 x 6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
ZnSO4 x 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 35 |
CuCl2 x 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace Elements
Dose: 10 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
Vitamin Solution
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.827255Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2598 |
| 2026-01-28T19:48:10.131974Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:16.525523+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:43.940424Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:03.498402Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009165 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.224035+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanogenium Medium" to "methanogenium_medium" for consistent matching |
| 2026-05-16T09:03:14.517850+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.785776+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.422794+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.358937+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:27.223599+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/15/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |