Overview
- External ontology
- TOGO:M2604 — Elusimicrobium minutum medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2604 Original URL: https://www.atcc.org/~/media/971A597A1CC2444AB3BA8C56538B92D4.ashx
Composition
flowchart LR
CultureMech_009172["elusimicrobium_minutum_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009172 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_Yeast_extract["Yeast extract"]
CultureMech_009172 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009172 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009172 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_009172 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_009172 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009172 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_KCl["KCl"]
CultureMech_009172 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_009172 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Fe_NH4_2_SO4_2_x_7_H2O["Fe(NH4)2(SO4)2 x 7 H2O"]
CultureMech_009172 --> ing9_Fe_NH4_2_SO4_2_x_7_H2O
CHEBI_131378["CHEBI:131378"]
ing9_Fe_NH4_2_SO4_2_x_7_H2O -.-> CHEBI_131378
ing10_MgCl2_x_7_H2O["MgCl2 x 7 H2O"]
CultureMech_009172 --> ing10_MgCl2_x_7_H2O
CHEBI_6636["CHEBI:6636"]
ing10_MgCl2_x_7_H2O -.-> CHEBI_6636
ing11_DI_Water["DI Water"]
CultureMech_009172 --> ing11_DI_Water
ing12_Glucose["Glucose"]
CultureMech_009172 --> ing12_Glucose
CHEBI_17234["CHEBI:17234"]
ing12_Glucose -.-> CHEBI_17234
ing13_Casamino_acids["Casamino acids"]
CultureMech_009172 --> ing13_Casamino_acids
ing14_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_009172 --> ing14_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing14_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing15_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009172 --> ing15_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing15_Carbon_dioxide_gas -.-> CHEBI_16526
ing16_Nitrogen_gas["Nitrogen gas"]
CultureMech_009172 --> ing16_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing16_Nitrogen_gas -.-> CHEBI_17997
ing17_Dithiothreitol__DTT_["Dithiothreitol (DTT)"]
CultureMech_009172 --> ing17_Dithiothreitol__DTT_
ing18_Na2MoO4_x_H2O["Na2MoO4 x H2O"]
CultureMech_009172 --> ing18_Na2MoO4_x_H2O
CHEBI_75215["CHEBI:75215"]
ing18_Na2MoO4_x_H2O -.-> CHEBI_75215
ing19_H3BO3["H3BO3"]
CultureMech_009172 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_MnCl2_x_4H2O["MnCl2 x 4H2O"]
CultureMech_009172 --> ing20_MnCl2_x_4H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_x_4H2O -.-> CHEBI_86368
ing21_CoCl2_x_6H2O["CoCl2 x 6H2O"]
CultureMech_009172 --> ing21_CoCl2_x_6H2O
CHEBI_35696["CHEBI:35696"]
ing21_CoCl2_x_6H2O -.-> CHEBI_35696
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009172 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_ZnSO4_x_7H2O["ZnSO4 x 7H2O"]
CultureMech_009172 --> ing23_ZnSO4_x_7H2O
CHEBI_32312["CHEBI:32312"]
ing23_ZnSO4_x_7H2O -.-> CHEBI_32312
ing24_CuCl2_x_2H2O["CuCl2 x 2H2O"]
CultureMech_009172 --> ing24_CuCl2_x_2H2O
CHEBI_86318["CHEBI:86318"]
ing24_CuCl2_x_2H2O -.-> CHEBI_86318
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.45 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 2 | Yeast extract | 0.2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.335 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Fe(NH4)2(SO4)2 x 7 H2O
Fe.7H2O.2H4N.2O4S 410.154 g/mol
|
2 G_PER_L |
CHEBI:131378
ferrous ammonium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MgCl2 x 7 H2O
2Cl.Mg 95.211 g/mol
|
2.75 G_PER_L |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | DI Water | 1980.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 970.0, 10.0, 1000.0] | |
| 13 |
Glucose
C6H12O6 180.156 g/mol
|
0.18 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 14 | Casamino acids | 0.2 G_PER_L | Properties: Undefined component | |
| 15 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Simple component, Defined component, Organic compound |
| 16 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 17 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 18 | Dithiothreitol (DTT) | 0.368 G_PER_L | Properties: Defined component, Simple component, Inorganic compound | |
| 19 |
Na2MoO4 x H2O
MoO4.2Na 205.916 g/mol
|
0.03 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
MnCl2 x 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
CoCl2 x 6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
ZnSO4 x 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
CuCl2 x 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Wolfe's Vitamin Solution
Dose: 10 G_PER_L
Role: Growth factor; Properties: Complex component
Trace Elements Solution SL-6
Dose: 10 G_PER_L
DTT Solution
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.843564Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2604 |
| 2026-01-28T19:48:38.258598Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:05:04.008103+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:35.955464Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:03.579082Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009172 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.308423+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Elusimicrobium minutum medium" to "elusimicrobium_minutum_medium" for consistent matching |
| 2026-05-16T09:03:31.411432+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:59.491509+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:38.078920+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:56.682003+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |